iProXProteomicsAn QinHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0012448000dr_qinan@163.comShanghai Jiao Tong University School of MedicineiProXfalseData-independent acquisition (DIA) based quantitative proteomic analysis of dermal fibroblast supernatant samplesWe collected fibroblasts derived from the foreskin of two healthy patients and cultured the cells in 7.5% HPL + DMEM medium. After in vitro expansion to passage 3 (P3), once the cells reached 80–90% confluency, the medium was replaced with an equal volume of fresh DMEM. The conditioned supernatant produced over a 3-day period was collected and subjected to proteomic sequencing to determine the protein composition of the fibroblast-derived supernatant.Fri Jun 27 00:00:00 GMT+01:00 2025PXD0655969606