iProXProteomicsDapeng LiHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0012727000dpli@scu.edu.cnSichuan UniversityiProXfalseSerum N-Glycoproteome Mapping Reveals Glycosite and Glycan Signatures in Type 2 Diabetes Mellitus Complicated with Coronary Heart DiseaseThis study investigated the glycoproteomic alterations associated with the development of CHD in patients with T2DM, with a particular focus on dual-layer changes in N-glycosylation sites (N-glycosites) and intact N-glycopeptides. Serum samples were analyzed using a hydrophilic interaction liquid chromatography (HILIC)-based MS method to perform comprehensive glycoproteomic profiling at both the site-specific glycopeptide and intact N-glycopeptide levels. This approach enabled the simultaneous identification of glycosylation sites and their attached glycan structures, offering high-resolution insights into glycan heterogeneity across glycoproteins. These findings advance our understanding of the molecular mechanisms underlying T2DM&CHD and show the promising value of glycoproteomics for identifying disease-specific biomarkers.Thu Jul 24 00:00:00 BST 2025PXD0665059606