iProXProteomicsJianrong XuMus Musculushttp://www.iprox.org/page/project.html?id=IPX0012747000jianrong.xu@shutcm.edu.cnShanghai University of Traditional Chinese MedicineiProXfalseDiscovery of TRPV1-Associated Molecular Chaperones via PafA-Mediated Proximity LabelingTo identify TRPV1-associated molecular chaperones, we applied a PafA-based proximity labeling system using both N- and C-terminal TRPV1 fusions. Three groups were co-transfected with BCCP-Pup(E) for in-cell biotinylation. Biotinylated proteins were validated by immunofluorescence and Western blot, followed by streptavidin pull-down and mass spectrometry.Tue Jul 29 00:00:00 GMT+01:00 2025PXD06669810090