{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Qiang Ding"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0013733000"],"submitter_email":["qding@tsinghua.edu.cn"],"submitter_affiliation":["Tsinghua University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Proximity labeling-based proteomic analysis of the HEV ORF1-associated replication complex under EIF4H deficiency","description":"This project explores the role of EIF4H in regulating the composition of the HEV ORF1-associated replication complex using proximity-dependent biotin labeling with TurboID. TurboID was inserted into a validated permissive site within the ORF1 coding region that allows insertion without disrupting ORF1 function. HEK293T wild-type and EIF4H-knockout cells expressing ORF1-TurboID were subjected to biotin labeling, streptavidin-based enrichment, and mass spectrometry analysis. Comparative proteomics identified EIF4H-dependent host factors associated with the HEV replication complex.","dates":{"publication":"Thu Oct 09 00:00:00 GMT+01:00 2025"},"accession":"PXD069279","cross_references":{"TAXONOMY":["9606"]}}