iProXProteomicsQiang DingHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0013733000qding@tsinghua.edu.cnTsinghua UniversityiProXfalseProximity labeling-based proteomic analysis of the HEV ORF1-associated replication complex under EIF4H deficiencyThis project explores the role of EIF4H in regulating the composition of the HEV ORF1-associated replication complex using proximity-dependent biotin labeling with TurboID. TurboID was inserted into a validated permissive site within the ORF1 coding region that allows insertion without disrupting ORF1 function. HEK293T wild-type and EIF4H-knockout cells expressing ORF1-TurboID were subjected to biotin labeling, streptavidin-based enrichment, and mass spectrometry analysis. Comparative proteomics identified EIF4H-dependent host factors associated with the HEV replication complex.Thu Oct 09 00:00:00 BST 2025PXD0692799606