iProXProteomicsNan ZhaoHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0014583000zn15877991832@outlook.comThe first Hospital of KunmingiProXfalseProteomics data for: Pan-Apoptotic Protein Signature as a Novel Diagnostic and Prognostic Biomarker for Intracerebral HemorrhageIntracerebral hemorrhage (ICH) remains a major cause of morbidity and mortality, with no rapid blood-based biomarkers available to assess secondary brain injury. This study aimed to identify a circulating pan-cell death protein signature for diagnosing and prognosing ICH. We prospectively enrolled 60 ICH patients and 60 age/sex-matched healthy controls, collecting plasma at defined time points after ICH onset. Using Olink® proteomics, we identified 13 apoptosis-related proteins with significant dysregulation. Four key proteins—TLR4, ALOX15, FTL, and BMF—were validated through ELISA and RT-qPCR, showing good diagnostic performance (AUCs 0.799–0.835). A multi-protein logistic model demonstrated excellent diagnostic accuracy (AUC = 0.955). These biomarkers correlated with clinical severity and prognosis, including hematoma volume and 90-day modified Rankin Scale (mRS). Additionally, animal models confirmed time-dependent upregulation of TLR4 in astrocytes. This pan-cell death protein signature provides valuable insights into ICH pathology and offers a promising tool for early diagnosis, risk stratification, and therapeutic targeting.Thu Dec 11 00:00:00 GMT 2025PXD0718569606