{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Junying Miao"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0014675000"],"submitter_email":["miaojy@sdu.edu.cn"],"submitter_affiliation":["Shandong university"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Exogenous Glutathione S-transferase Mu 2 (GSTM2) supplementation has emerged as a promising strategy to counteract aging. However, approaches to enhance endogenous GSTM2 expression remain underexplored. Here, we identify HCY-NBD, an SO<sub>2</sub>-targeting small molecular that binds GSTM2 and stabilizes GSTM2 protein levels under high-glucose (HG)-induced vascular endothelial senescence. Mechanistically, HCY-NBD promotes sulfenylation at Cys174 of GSTM2 and inhibits its K48-linked ubiquitination at this residue, thereby stabilizing GSTM2 protein. In cellular studies, we observe that HCY-NBD upregulates GSTM2 and inhibits HG-induced senescence and calcification in vascular endothelial cells. Consistent with this, in vivo administration of HCY-NBD in db/db mice increases GSTM2 levels and mitigates senescence and calcification in the thoracic aorta. Collectively, our findings demonstrate that HCY-NBD inhibits HG-induced vascular senescence and calcification by stabilizing GSTM2 protein levels via enhancing Cys174 sulfenylation and suppression of site-specific ubiquitination-mediated degradation. Here, we first develop a new strategy to enhance endogenous GSTM2 and provide a novel therapeutic strategy for the prevention and treatment of vascular aging."],"pubmed_title":["Small molecule HCY-NBD stabilizes GSTM2 via cys174 sulfenylation to attenuate high glucose induced endothelial cell senescence and calcification."],"pubmed_authors":["Zhang Yangyang Y, Dong Xinyu X, Yan Xiaomeng X, Zhao Congyao C, Chi Xiaohui X, Zhao Baoxiang B, Miao Junying J, Hong Fanzhen F, Lin Zhaomin Z"],"additional_accession":[]},"is_claimable":false,"name":"Identification of ubiquitination sites on the GSTM2 protein","description":"The human GSTM2 protein was enriched, followed by mass spectrometry to identify its ubiquitination sites.","dates":{"publication":"Mon Dec 15 00:00:00 GMT 2025"},"accession":"PXD071988","cross_references":{"TAXONOMY":["9606"],"pubmed":["41637880"]}}