{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Cao Yang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0014787000"],"submitter_email":["caoyangunion@hust.edu.cn"],"submitter_affiliation":["Union Hospital, Tongji Medical College, Huazhong University of Science and Technology"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication. However, the mechanisms governing their degradation remain poorly understood. In this study, we demonstrated that EVs are predominantly degraded via the lysosomal pathway. Mechanistically, MAP1LC3B recognizes SNX18 on the surface of endosome-escaped EVs to facilitate their sorting into the autolysosomal pathway for degradation. Leveraging this mechanism, we optimized the lysosomal sorting efficiency of EVs by surface display of LIR motifs and constructed an EV-based targeted protein degradation nanoplatform. The EV-based nanoplatform is highly modular and can be combined with monoclonal antibodies in a plug-and-play manner. It demonstrated remarkable efficiency and selectivity in degrading EGFR, PD-L1, and VEGF. Moreover, the nanoplatform demonstrated multi-targeting capability by simultaneously degrading EGFR and VEGF. Our findings uncover a previously unrecognized mechanism of EVs degradation and provide a novel strategy to harness the EVs degradation machinery as a nature-inspired nanoplatform for the degradation of multiple targeted proteins."],"pubmed_title":["A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway."],"pubmed_authors":["Tong Bide B, Wang Yulei Y, Wei Junyu J, Ou Zixuan Z, Liang Huaizhen H, Lei Jie J, Zhu Dingchao D, Xu Hanpeng H, Tan Lei L, Liao Zhiwei Z, Yang Cao C"],"additional_accession":[]},"is_claimable":false,"name":"A nature-inspired nanoplatform for multiple targeted proteins degradation via autophagy-lysosome pathway","description":"This project primarily investigates the interactome of SNX18, a key mediator protein in the degradation of extracellular vesicles (EVs), as well as the degradation efficiency of EGFR and PDL1 using an SNX18-LIR motif-based overexpression platform for targeted protein degradation.","dates":{"publication":"Mon Dec 22 00:00:00 GMT 2025"},"accession":"PXD072326","cross_references":{"TAXONOMY":["9606"],"pubmed":["41932333"]}}