iProXProteomicsZehong DingManihot Esculentahttp://www.iprox.org/page/project.html?id=IPX0014780000zehong3141@sina.comInstitute of Tropical Bioscience and BiotechnologyiProXLysine acetylation (Kac), 2-hydroxyisobutyrylation (Khib), and malonylation (Kma) represent three recently identified posttranslational modifications (PTMs) that regulate plant development and stress resilience. Herein, we constructed the first global proteomic atlas of Kac, Khib, and Kma modifications in developing cassava roots, identifying 11,253 Kac, 18,326 Khib, and 4068 Kma sites across 5165, 4832, and 1815 proteins, respectively. The PTM-modified proteins were involved in sucrose/starch metabolism, glycolysis/gluconeogenesis, pentose phosphate pathway, TCA cycle, and lignin biosynthesis, with the majority exhibiting multiple PTM co-occurrence. Hundreds of modified proteins associated with stress response, hormone metabolism, and transcription factors were also identified, of which a few proteins displayed significant type-specific modification preferences. Finally, the regulatory roles of Kac-, Khib-, and Kma-modified proteins in root development and stress responses were discussed, leading to a proposed mechanistic model for PTM-mediated regulation in cassava. These findings provide novel insights for elucidating the molecular mechanisms of PTM-driven regulation in plants.Deciphering the Atlas of Protein Acetylation, 2-Hydroxyisobutyrylation, and Malonylation in Developing Cassava Roots.Fu Lili L, Yan Yan Y, Huo Kaisen K, Tie Weiwei W, Yang Jinghao J, Tan Deguan D, Hu Wei W, Ding Zehong ZfalseThe atlas of protein acetylation, 2-hydroxyisobutyrylation, and malonylation in developing cassava rootsIn this study, we constructed the first global proteomic atlas of Kac, Khib, and Kma modifications in developing cassava roots, identifying 11,253 Kac, 18,326 Khib, and 4,068 Kma sites across 5,165, 4,832, and 1,815 proteins, respectively. Comparative sequence analysis revealed conservation of Kac and Khib sites in histones across cassava, sugarcane, and rice, with higher sequence conservation observed in histone H2A and H4 compared to H2B. Functional enrichment demonstrated that PTM-modified proteins were predominantly involved in sucrose/starch metabolism, glycolysis/gluconeogenesis, pentose phosphate pathway, TCA cycle, pyruvate metabolism, and lignin biosynthesis, with the majority exhibiting multiple PTM co-occurrence. In addition, hundreds of modified proteins associated with stress response, hormone metabolism, and transcription factors were also identified, of which a few proteins displayed significant type-specific modification preferences.Tue Dec 23 00:00:00 GMT 2025PXD072330398341636360