{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yi Nan"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0015725000"],"submitter_email":["20080011@nxmu.edu.cn"],"submitter_affiliation":["Ningxia Medical University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["18β-Glycyrrhetinic acid (18β-GRA), a major bioactive component of licorice, exhibits recognized biological activities in various tumor models. However, its cellular effects and underlying molecular mechanisms in tongue squamous cell carcinoma (TSCC) are not well characterized. This study aimed to investigate the molecular mechanisms of 18β-GRA against TSCC by integrating quantitative proteomics with network pharmacology. The CCK-8 assay was used to assess cell viability. Cell-cycle distribution and apoptosis were analyzed by flow cytometry. Intracellular Reactive Oxygen Species (ROS) levels were detected using DCFH-DA staining. Tandem Mass Tag (TMT)-based quantitative proteomics was employed to identify differentially expressed proteins, followed by functional enrichment and subcellular localization analyses. Western blotting was performed to examine the expression of proteins associated with apoptosis, autophagy, and the PI3K/AKT pathway. Network pharmacology was applied to predict potential targets of 18β-GRA. Common targets shared with TSCC were mapped onto a protein-protein interaction network, and molecular docking was used to evaluate the binding affinity of 18β-GRA to these overlapping proteins. 18β-GRA significantly inhibited SCC-9 cell viability in a dose- and time-dependent manner and induced G0/G1 phase arrest. The percentages of apoptotic cells and intracellular ROS levels increased with higher concentrations of 18β-GRA. Proteomic analysis revealed widespread alterations in pathways involved in metabolism, cellular structure, apoptosis, and autophagy. Western blotting confirmed upregulation of Bax, downregulation of Bcl-2, an increased LC3-II/LC3-I ratio, decreased p62 expression, and reduced phosphorylation of AKT1. Network pharmacology identified six intersection targets between 18β-GRA and TSCC, which were enriched in metabolic and cancer-related pathways including the PI3K-Akt pathway. Molecular docking suggested stable binding interactions between 18β-GRA and all six candidate proteins. 18β-GRA exerts multi-faceted effects on SCC-9 cells, including growth inhibition, cell-cycle arrest, apoptosis induction, oxidative stress enhancement, and modulation of autophagy markers. The integrated proteomic and computational approach implicates several key pathways and protein targets in the anti-TSCC activity of 18β-GRA, providing a broader molecular context for its mechanistic understanding."],"pubmed_title":["Mechanistic insights into 18β-glycyrrhetinic acid-induced apoptosis in SCC-9 cells revealed by TMT proteomics and network pharmacology."],"pubmed_authors":["Fan Hongli H, Yuan Daichang D, Nan Yi Y"],"additional_accession":[]},"is_claimable":false,"name":"TMT-based proteomic analysis of SCC-9 cells following treatment with 18β-Glycyrrhetinic acid","description":"Project Description: TMT-Based Quantitative Proteomic Profying of SCC-9 Cells Treated with 18β-Glycyrrhetinic Acid This project employs Tandem Mass Tag (TMT) multiplexed quantitative proteomics to comprehensively analyze changes in the global protein expression profile of human tongue squamous cell carcinoma (SCC-9) cells following treatment with the bioactive compound 18β-Glycyrrhetinic Acid (18β-GA). The experiment was designed to compare the proteomes of SCC-9 cells exposed to a physiologically relevant concentration of 90uM 18β-GA against the negative control (NC) wells received medium containing 0.05% DMSO for 48h.After the intervention period, cells were harvested, and proteins were extracted, digested, and labeled using a TMT reagent kit. The pooled, labeled peptides were then fractionated and analyzed by liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-MS/MS). The primary goal of this study is to identify differentially expressed proteins induced by 18β-GA, which may reveal its molecular mechanisms of action in oral cancer cells. This includes potential effects on key signaling pathways, cellular processes such as proliferation, apoptosis, migration, and metabolism, and the discovery of novel protein targets. The generated dataset provides a system-wide view of the protein-level response to 18β-GA, serving as a valuable resource for hypothesis generation and further functional validation in cancer research.","dates":{"publication":"Tue Feb 10 00:00:00 GMT 2026"},"accession":"PXD074323","cross_references":{"TAXONOMY":["9606"],"pubmed":["42140987"]}}