iProXProteomicsMingliang YeHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0015965000mingliang@dicp.ac.cnDalian Institute of Chemical Physics, Chinese Academy of SciencesiProXfalseTEO-GalNAc: Tag-free Chemoenzymatic Enrichment and Quantitative Mapping of Tn/T Antigen Sialylation Occupancy via Ketone-Oxime ChemistryTEO-GalNAc is a chemoenzymatic strategy for tag-free enrichment and quantitative mapping of Tn/T O-GalNAc glycopeptides. Ketone-modified CMP-Sia is transferred by ST6GalNAc1 for selective tagging, followed by reversible oxime capture using hydroxylamine beads. Mild acid release enables tag-free recovery and quantitative assessment of sialylation occupancy on core GalNAc residues in complex samples.Wed Mar 04 00:00:00 GMT 2026PXD0752399606