{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Hong Liu"],"species":["Oryctolagus Cuniculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0016072000"],"submitter_email":["liuhongzef@263.net"],"submitter_affiliation":["Shanghai Jiao Tong University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<h4>Introduction</h4>Human amniotic epithelial cell-derived extracellular vesicles (hAEC-EVs) have shown therapeutic potential in corneal injury repair; however, the underlying molecular mechanisms, particularly those related to extracellular matrix (ECM) remodeling, remain incompletely understood.<h4>Methods</h4>A proteomic and bioinformatic strategy was applied to analyze ECM-related molecular alterations in corneal tissues following alkali injury and hAEC-EV treatment. Differentially expressed ECM-related genes were identified and subjected to pathway enrichment, protein-protein interaction, and immune infiltration analyses. To enhance experimental rigor, key findings were validated at both the transcript and protein levels using quantitative real-time PCR and Western blotting. In addition, <i>in vitro</i> functional assays were performed to assess the effects of hAEC-EVs on corneal epithelial and stromal cell proliferation and migration.<h4>Results</h4>hAEC-EV treatment significantly upregulated ECM-stabilizing molecules, including A2M, LAMA1, and VIT, while downregulating the injury- and inflammation-associated protease CTSB at both mRNA and protein levels. Enrichment analyses revealed that hAEC-EVs modulate ECM-receptor interaction pathways and cell-ECM communication. Functional assays confirmed that hAEC-EVs directly enhance the proliferation and migration of human corneal epithelial cells and human corneal stromal cells. Immune infiltration analysis further suggested that hAEC-EVs reshape the corneal immune microenvironment toward a repair-permissive state.<h4>Conclusion</h4>Through integrated proteomic, bioinformatic, protein-level validation, and functional analyses, this study demonstrates that hAEC-EVs promote corneal repair by coordinating ECM remodeling, regulating key signaling networks, and modulating immune responses, providing mechanistic support for their therapeutic application in corneal injuries."],"pubmed_title":["Targeted proteomic and bioinformatic investigation of extracellular matrix remodeling in hAEC-EV-mediated corneal repair."],"pubmed_authors":["Hu Shuqin S, Qiu Ting T, Liu Hong H"],"additional_accession":[]},"is_claimable":false,"name":"Targeted proteomic and  bioinformatic investigation of  extracellular matrix remodeling in  hAEC-EV-mediated corneal repair","description":"Human amniotic epithelial cell-derived extracellular vesicles  (hAEC-EVs) have shown therapeutic potential in corneal injury repair; however,  the underlying molecular mechanisms, particularly those related to extracellular  matrix (ECM) remodeling, remain incompletely understood.","dates":{"publication":"Wed Mar 11 00:00:00 GMT 2026"},"accession":"PXD075526","cross_references":{"TAXONOMY":["9986"],"pubmed":["41929428"]}}