{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Zhiyuan Chen"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0016392000"],"submitter_email":["drchenzy@whu.edu.cn"],"submitter_affiliation":["Renmin Hospital of Wuhan University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Immunoprecipitation-mass spectrometry (IP-MS) of USP15-interacting proteins in 786-O cells","description":"786-O cells were lysed in IP buffer and processed for immunoprecipitation with anti-USP15 or normal rabbit IgG for 6 h at 4 °C. The immune complexes were incubated with Pierce Protein A/G Magnetic Beads (Thermo Scientific) overnight at 4 °C and washed with IP buffer. After SDS-PAGE and Coomassie brilliant blue staining, the gel was cut into sections and digested with trypsin. For each sample, 2 µg of total peptides were separated and analyzed with a nano-UPLC (nanoElute2) coupled to a timsTOF Pro2 mass spectrometer (Bruker) with a nano-electrospray ion source. Then the raw MS files were processed using PaSER software (Version 2023) and the built-in DDA-ProluCID-GPU search engine.","dates":{"publication":"Thu Mar 26 00:00:00 GMT 2026"},"accession":"PXD076226","cross_references":{"TAXONOMY":["9606"]}}