{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Hucheng Zhang"],"species":["Cordyceps Militaris Cm01"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0016387000"],"submitter_email":["zhanghchbj@163.com"],"submitter_affiliation":["Beijing Polytechnic University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["The growth-specialized metabolism trade-off limits fungal natural product production. Here, we investigated cordycepin overproduction in <i>Cordyceps militaris</i> high-yield GYS60 and low-yield GYS80 via comparative proteomics, Post-Translational Modification (PTM) mapping, and metabolomics. We identified an acetyl-CoA-gated checkpoint centered on O-methyltransferase CCM_06472, whose activity is modulated by Lys123 acetylation and Ser34 phosphorylation in a manner consistent with activation and inhibition. GYS60 hyperactivates the tricarboxylic acid (TCA) cycle and β-oxidation to generate a 4.1-fold acetyl-CoA surplus, 62% of which is channeled into cordycepin synthesis. A single K123Q acetylation-mimetic mutation boosted cordycepin titers by >4-fold in wild-type strains. This acetyl-CoA checkpoint reveals PTM-gated flux allocation as a key regulatory mechanism, providing a minimal-intervention strategy for engineering fungal cell factories."],"pubmed_title":["An Acetyl-CoA-Gated Metabolic Checkpoint Links Precursor Supply to Cordycepin Biosynthesis in &lt;i&gt;Cordyceps militaris&lt;/i&gt;."],"pubmed_authors":["Zhang Hucheng H, Yang Dongqing D, Yang Guowei G, Luo Shuai S, Lu Peng P, Xie Guoli G, Song Yapeng Y, Yang Jun J, Liu Linying L, Fan Haitao H, Lao Wenyan W, Vriesekoop Frank F"],"additional_accession":[]},"is_claimable":false,"name":"Integration of genome, transcriptome and proteome of cordycepin  high producing strains","description":"Integration of genome, transcriptome and proteome of cordycepin  high producing strains","dates":{"publication":"Sat Mar 28 00:00:00 GMT 2026"},"accession":"PXD076340","cross_references":{"TAXONOMY":["983644"],"pubmed":["42042030"]}}