iProXProteomicsYuping GongHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0016422000gong2025@wchscu.edu.cnWest China HospitaliProXfalseProteomic profiling of bone marrow aspirate (BMA) samples collected pre-VD-CAG induction and post-induction VD-CAG-treated non-M3 AMLProteomic profiling was performed by Novogene (Beijing, China), using data-independent acquisition (DIA) proteomics on paired bone marrow aspirate samples from 5 non-M3 AML patients before and after VD-CAG treatment. All patients achieved composite complete response (CRc) after 1 cycle of VD-CAG induction. Protein extraction, quantification, enzymatic digestion with trypsin (allowing up to 2 missed cleavage sites), desalting, and fractionation were conducted following standard protocols to ensure data quality at each step. Peptides were separated using nano-LC on a Vanquish Neo system and analyzed on an Orbitrap Astral high-resolution mass spectrometer, which features a quadrupole for precursor ion selection, an Orbitrap analyzer for high-dynamic-range HRAM spectra, and an Astral analyzer for high-sensitivity, up to 200 Hz HRAM spectral acquisition.Mon Mar 30 00:00:00 BST 2026PXD0763489606