iProXProteomicsNanxi WangHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0016542000nanxi.wang@njucm.edu.cnNanjing University of Chinese MedicineiProXfalseGenetic code expansion enables site-specific acetylation to reprogram Lunasin toward BRD4 targetingLunasin is a plant-derived peptide with moderate anticancer activity but has limited potency and undefined molecular targets. To enhance its functional properties, we developed a site-specifically acetylated variant (Lunasin-K29ac) via genetic code expansion. To characterize the molecular interactions underlying its enhanced activity, we performed interactome profiling of a control (pET-22b-CBD), wild-type Lunasin, and Lunasin-K29ac in HT-29 cells using His pull-down MS.Tue Apr 07 00:00:00 GMT+01:00 2026PXD0767199606