iProXProteomicsJibin SunCorynebacterium Glutamicum Atcc 13032http://www.iprox.org/page/project.html?id=IPX0016580000sun_jb@tib.cas.cnTianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesiProXfalseComprehensive O-Phosphoproteomic Landscape of Corynebacterium glutamicumIn this study, we implemented a highly robust workflow for O-phosphopeptides enrichment utilizing titanium-based immobilized metal affinity chromatography (Ti-IMAC). By integrating an offline reversed-phase prefractionation strategy and a combination of Electron-Transfer/Higher-Energy Collision Dissociation (EThcD) and Higher-Energy Collision Dissociation (HCD) mass spectrometry fragmentation techniques, we established the most comprehensive phosphoproteomic dataset for this species to our knowledge.Thu Apr 09 00:00:00 GMT+01:00 2026PXD076840196627