iProXProteomicsCuihong WanChlamydomonas Reinhardtiihttp://www.iprox.org/page/project.html?id=IPX0016859000ch_wan@mail.ccnu.edu.cnCentral China Normal UniversityiProXfalseChlamydomonas reinhardtii ciliary membrane proteomeThe cell membrane fraction of Chlamydomonas cw15 and the ciliary membrane fraction of Chlamydomonas cc125 were extracted by aqueous two-phase extraction and sucrose density gradient centrifugation, respectively. The membrane fractions were dissolved in 8 M urea to obtain plasma membrane proteins (PM) and ciliary membrane proteins (FM). TMT labeling reagents (126-129) were used to label the two biological replicates of each fraction separately. The labeled peptides were fractionated into 10 fractions by liquid chromatography, followed by mass spectrometry analysis.Sun Apr 26 00:00:00 BST 2026PXD0776433055