iProXProteomicsMingnan QuGlycine Maxhttp://www.iprox.org/page/project.html?id=IPX0016878000qmn@yzu.edu.cnCollege of AgricultureiProXfalseRaw mass spectrometry data for the identification of GmGNAT10 as a key acetyltransferase mediating acetylation modification of Rubisco in soybeanRibulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the core rate-limiting enzyme of photosynthetic carbon metabolism, is regulated by acetylation, yet its upstream acetyltransferases remain largely uncharacterized. Here, we identified a novel soybean acetyltransferase GmGNAT10, which interacts with Rubisco and other photosynthetic enzymes via IP-MS/MS interactomics. Quantitative acetylomics under fluctuating light revealed dramatic genotypic differences in acetylation levels of key RbcL lysine residues (K23, K175, K236) between wild-type and GmGNAT10 mutants. Our findings expand the mechanistic understanding of chloroplast non-histone acetylation in fine-tuning photosynthetic carbon metabolism.Tue Apr 28 00:00:00 GMT+01:00 2026PXD0777053847