iProXProteomicsXuefanYuHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0016967000xuefan@jlu.edu.cnThe First Affiliated Hospital of Jilin UniversityiProXfalseNovel phenotypes of immune-mediated necrotizing myopathy identified independent of myositis-specific antibody specificity that improve prognostic stratificationImmune-mediated necrotizing myopathy (IMNM) is a severe autoimmune myopathy with high clinical heterogeneity. Current classification relies on myositis-specific antibodies (MSA: anti-HMGCR, anti-SRP) but fails to explain differences in manifestations, interstitial lung disease (ILD) incidence, and survival. We enrolled 133 IMNM patients, used unsupervised machine learning (MSA-independent) to identify three phenotypes: Phenotype 1 (56.4%, muscle weakness, favorable prognosis), Phenotype 2 (10.5%, high muscle damage indicators, intermediate prognosis), Phenotype 3 (33.1%, high ILD/mortality, poor prognosis). Label-free DIA quantitative proteomics on these phenotypes and noninflammatory controls revealed distinct protein expression patterns, providing molecular evidence for IMNM subtyping, mechanism exploration, and prognosis evaluation.Wed May 06 00:00:00 BST 2026PXD0781549606