iProXProteomicsShunji JiaHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0017066000jiasj@genetics.ac.cnInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesiProXfalseIdentification of regulatory protein networks of SEC14L2 in 293T cellsFor mass spectrometry analysis of SEC14L2 and STMN1 binding partners, 293T cells expressing SEC14L2-Flag or STMN1-Flag were lysed in NP-40 lysis buffer. The cytosolic proteins were collected by centrifugation and incubated with anti-Flag beads for 2 h, followed by five washes with NP-40 lysis buffer. Bound proteins were then eluted using 0.1 M glycine, subjected to mass spectrometry analysis.Sat May 09 00:00:00 BST 2026PXD0782119606