iProXProteomicsGuojun ChenHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX00171100001009205828@qq.comThe First Affiliated Hospital of Chongqing Medical UniversityiProXfalseDIA-Based Quantitative Proteomic Dataset of SH-SY5Y Cells Overexpressing Wild-Type and SAP-Domain Deleted MKL2This project provides a comprehensive quantitative proteomics dataset generated from human neuroblastoma SH-SY5Y cells to investigate the regulatory roles of MKL2 and its SAP domain. The study involves three experimental groups with three biological replicates each (9 samples in total). SH-SY5Y cells were transiently transfected with an empty vector (Control), wild-type MKL2 (Full-length), or a mutant MKL2 lacking the SAP domain. The raw mass spectrometry files are named as follows: (1) Control group: vector1-3.raw; (2) Wild-type MKL2 group: M_FLOE1-3.raw; (3) SAP-domain deleted MKL2 group: MSAPOE1-3.raw. Cells were harvested 48 to 72 hours post-transfection, and total proteins were extracted using SDT lysis buffer (4% SDS, 100 mM DTT, 100 mM Tris-HCl, pH 8.0). Protein digestion was performed using the Filter-Aided Sample Preparation (FASP) method. The resulting peptides were analyzed on an Orbitrap Astral mass spectrometer coupled with a Vanquish Neo UHPLC system in data-independent acquisition (DIA) mode (survey scan: 380–980 m/z; MS/MS scan: 150–2000 m/z). Raw data files were initially processed using DIA-NN software (version 1.8.1) searched against the UniProtKB Homo sapiens database for quality control and protein identification.Mon May 11 00:00:00 BST 2026PXD0782289606