iProXProteomicsLikun SunKlebsiella Sp.http://www.iprox.org/page/project.html?id=IPX0017126000sunlk_baby@126.comGansu Agricultural UniversityiProXfalsemulti-omics analysis of zinc tolerance and the maintenance of high-efficiency denitrification in Klebsiella sp. WH-E under Zn²⁺ stressThis study systematically evaluated the denitrification performance and tolerance mechanisms of the highly efficient heterotrophic nitrifying–aerobic denitrifying strain Klebsiella sp. WH-E under Zn²⁺ stress. Under 100 mg/L Zn²⁺ stress, the heterotrophic nitrification and aerobic denitrification capabilities of Klebsiella sp. WH-E resulted in an ammonia nitrogen removal rate of 95.53% and a nitrate nitrogen removal rate of 74.00%. and the nitrite nitrogen removal rate was 72.75%. The activities of the key denitrification enzymes NAR and NIR remained largely stable, and the accumulation of NO₃⁻-N and NO₂⁻-N during nitrogen transformation was limited. Multi-omics analysis revealed that Zn²⁺ stress significantly upregulated nitrogen metabolism-related genes (narG, nirB) and metal homeostasis regulatory genes (zntA, arsR, czcC), while simultaneously regulating ABC transporters and two-component systems to maintain intracellular Zn²⁺ homeostasis. The abundance of sulfur metabolism and antioxidant-related metabolitWed May 13 00:00:00 GMT+01:00 2026PXD078319576