{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Guohong Li"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0017199000"],"submitter_email":["liguohong@whu.edu.cn"],"submitter_affiliation":["Wuhan University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"FLAG-IP–MS profiling of SPINDOC interactome using a HeLa–Tet-On–FLAG-SPINDOC-GFP cell line","description":"HeLa–Tet-On–FLAG-SPINDOC-GFP cells were established for inducible expression and affinity purification. FLAG-SPINDOC-GFP expression was first induced by doxycycline treatment. Subsequently, cells were either left unsynchronized or arrested at the G2/M phase by nocodazole treatment. Efficient expression and enrichment of FLAG-SPINDOC-GFP were confirmed by FLAG co-immunoprecipitation. Immunoprecipitates were then subjected to mass spectrometry analysis to identify SPINDOC-associated proteins, particularly under mitotic conditions.","dates":{"publication":"Fri May 15 00:00:00 GMT+01:00 2026"},"accession":"PXD078443","cross_references":{"TAXONOMY":["9606"]}}