iProXProteomicsZhenye YangHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0017437000zhenye@ustc.edu.cnUniversity of Science and Technology of ChinaiProXfalseMitochondrial proteomics in different cell cycle phasesFor interphase synchronization, HeLa cells were synchronized with 2 mM thymidine for 19 h, and these interphase cells were harvested. For mitotic synchronization, HeLa cells were treated with 2 mM thymidine for 19 h, followed by a 9 h release in fresh medium, allowing the cells to enter the mitotic phase. Mitotic cells were then harvested by mitotic shake-off. Crude mitochondria were subsequently isolated from both the harvested interphase and mitotic cells. The mitochondrial samples were directly subjected to LC-MS/MS analysis.Tue May 26 00:00:00 BST 2026PXD0788799606