{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yingping Zhuang"],"species":["Staphylococcus Aureus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0017381000"],"submitter_email":["ypzhuang@ecust.edu.cn"],"submitter_affiliation":["School of Biotechnology, East China University of Science and Technology"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Energy-translation coupling limits yeast anaerobic growth","description":"This study employs yeast as a model system to thoroughly investigate the differences in regulatory mechanisms under various nutrient limitation conditions, utilizing a systems biology approach integrated with techniques such as multi-omics analysis.To obtain a comprehensive quantitative landscape of protein expression in (human lung adenocarcinoma), we performed TMT 6-plex–based comparative proteomic analysis. Tumour and adjacent non-tumour tissues (n = 6 pairs) were pulverised under liquid nitrogen and total proteins extracted by phenol–ammonium acetate/methanol precipitation. After reduction, alkylation and acetone precipitation, 50 µg proteins per sample were digested in-solution with trypsin (1:50, w/w, 37 °C, 16 h). Peptides were labelled with TMT 6-plex reagents, pooled and fractionated by high-pH reversed-phase chromatography on an Agilent 1100 HPLC (Zorbax Extend-C18, 15 fractions). LC-MS/MS was carried out on a Q Exactive HF-X (Thermo) in DDA mode. Quantitative analysis was performed with Proteome Discoverer 2.4 using a 1 % FDR threshold at PSM and protein levels. The dataset provides relative abundance information for > 6 000 protein groups across tumour and control tissues, serving as a resource for biomarker and pathway studies in lung adenocarcinoma.","dates":{"publication":"Thu May 28 00:00:00 BST 2026"},"accession":"PXD078963","cross_references":{"TAXONOMY":["1280"]}}