iProXProteomicsXianqun FanMus Musculushttp://www.iprox.org/page/project.html?id=IPX0017527000fanxq@sjtu.edu.cnShanghai Ninth People's Hospital, Shanghai Jiao Tong University School of MedicineiProXfalseTRMT61A-mediated tRNA methylation regulates Th17 differentiation via a glycolysis–lactylation axis in autoimmune inflammationTo characterize the global landscape of lysine lactylation (Kla) in Th17 cells, we cultured naïve CD4⁺ T cells isolated from Trmt61a-cKO and WT mice under Th17-polarizing conditions for 96 hours. At least 1 × 10⁷ cells per sample were harvested for proteomic analysis. Following tryptic digestion, Kla-modified peptides were enriched using anti-Kla antibodies and analyzed by LC–MS/MS.Mon Jun 01 00:00:00 BST 2026PXD07916410090