{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Anwen Liu"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0017922000"],"submitter_email":["awliu666@163.com"],"submitter_affiliation":["The Second Affiliated Hospital of Nanchang University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Cardiac Lysine Succinylome Profiling of Wild-Type and SIRT5-Deficient Mice at 4 Months after Irradiation","description":"This study aimed to investigate the role of SIRT5 in regulating the cardiac succinylome following radiation exposure. The experiment utilized a cohort of six mice, comprising three SIRT5-knockout (SIRT5-KO) and three wild-type (WT) mice. All subjects received a single dose of thoracic radiation. Four months post-irradiation, cardiac tissues were collected. Quantitative proteomic analysis of succinylation modifications was then performed. Specifically, proteins were extracted from the heart tissues, digested with trypsin, and the resulting peptides were subjected to immunoaffinity enrichment using a high-specificity anti-succinyllysine antibody. The enriched succinylated peptides were analyzed using a timsTOF mass spectrometer coupled with nano-liquid chromatography in a 4D-data-independent acquisition (4D-FastDIA) mode. This advanced method provided high-depth, quantitative profiling of succinylation sites. The raw data were processed to identify and quantify lysine succinylation sites, enabling a comparative","dates":{"publication":"Mon Jun 29 00:00:00 BST 2026"},"accession":"PXD080310","cross_references":{"TAXONOMY":["10090"]}}