{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yihan Tian"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018147000"],"submitter_email":["2725353277@qq.com"],"submitter_affiliation":["Jilin University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"IP-MS analysis of PHEV nsp3/nsp4 host protein interactome in HEK293T cells","description":"This study investigates host protein interactomes of PHEV non-structural proteins nsp3 and nsp4 using affinity purification coupled with mass spectrometry (AP-MS). HEK293T cells were transiently transfected with HA-tagged empty vector, HA-tagged PHEV nsp3, or HA-tagged PHEV nsp4 constructs. After 48 hours post-transfection, cells were lysed and HA immunoprecipitation was performed to enrich interacting proteins. The immunoprecipitated complexes were separated by SDS-PAGE, and gel bands were excised for in-gel trypsin digestion followed by LC-MS/MS analysis to identify host protein interaction partners. This dataset provides a resource for understanding host–virus protein interaction networks of PHEV non-structural proteins.","dates":{"publication":"Fri Jul 03 00:00:00 GMT+01:00 2026"},"accession":"PXD080524","cross_references":{"TAXONOMY":["9606"]}}