{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Lian Ma"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018185000"],"submitter_email":["malian8965@sina.com"],"submitter_affiliation":["West China Second Hospital of Sichuan University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Advantages of Pooled-Donor Culture of Foreskin Mesenchymal Stromal Cells for Extracellular Vesicle Production: Insights from Multi-Omics Analysis","description":"This project characterizes the proteomic landscape of small extracellular vesicles (sEVs) derived from human adipose-, foreskin-, and umbilical cord-derived mesenchymal stromal cells (MSCs) cultured under single-donor or pooled-donor conditions in conventional 2D culture and 3D microcarrier-based bioreactors. DIA-based quantitative proteomics was used to determine how donor pooling and 3D culture reshape sEV cargo composition, reduce batch-level heterogeneity, and establish conserved protein signatures across tissue sources. The dataset identifies manufacturing-responsive molecular programs related to mitophagy, immune communication, phagocytosis, membrane repair, wound healing, and mitochondrial metabolism. Foreskin-derived MSC-sEVs showed the strongest response to pooled-donor and 3D culture conditions, supporting their potential as a platform for engineered immuno-mitochondrial and reparative vesicle products.","dates":{"publication":"Sun Jul 05 00:00:00 GMT+01:00 2026"},"accession":"PXD080621","cross_references":{"TAXONOMY":["9606"]}}