iProXProteomicsXianzhong ChengHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0018238000cxxxxcyd@gmail.comThe Affiliated Cancer Hospital of Nanjing Medical UniversityiProXfalseTumor-Derived Extracellular Vesicle Proteomics Enables Precise Ovarian Cancer Liquid BiopsyOvarian cancer (OC) remains one of the most lethal gynecological malignancies, and effective non-invasive biomarkers for early diagnosis, postoperative monitoring, and prognosis assessment are still limited. Serum CA125, the current standard biomarker for OC, has limited diagnostic accuracy, particularly in early-stage disease. Cancer-derived extracellular vesicles (EVs) carry tumor-specific proteomic information and represent promising biomarkers; however, their sensitive and specific isolation from complex biological fluids remains challenging. In this project, we developed BP-EVlent, a novel approach that combines polyethylene glycol (PEG)-based precipitation with CA125 immunoaffinity capture to precisely isolate plasma CA125-positive EVs from patients with OC. The performance of BP-EVlent was evaluated using data-independent acquisition mass spectrometry (DIA-MS)-based proteomic analysis of plasma samples from healthy controls (HC), preoperative OC patients (OC-pre), and postoperative OC patients (OC-postMon Jul 13 00:00:00 GMT+01:00 2026PXD0809669606