{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Honglin Wang"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018390000"],"submitter_email":["Honglin.wang@sjtu.edu.cn"],"submitter_affiliation":["Shanghai Jiao Tong University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"HSD17B4 interactome in mouse keratinocytes reveals association with splicing factors and RNA processing","description":"This project aims to elucidate the molecular mechanism by which HSD17B4 regulates CYP7B1 expression in mouse keratinocytes (mKCs). To identify proteins that interact with HSD17B4, we performed co-immunoprecipitation (Co-IP) using an HSD17B4-specific antibody, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The raw mass spectrometry data were acquired and processed to identify HSD17B4-interacting proteins. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the HSD17B4 interactome revealed a significant overrepresentation of proteins involved in RNA splicing and RNA processing. These findings suggest that HSD17B4 may regulate CYP7B1 expression through interactions with splicing factors, pointing to a previously unrecognized nuclear function of HSD17B4 beyond its enzymatic activity. This project contains the MS data for the interacting protein of HSD17B4 we identified.","dates":{"publication":"Thu Jul 16 00:00:00 GMT+01:00 2026"},"accession":"PXD081170","cross_references":{"TAXONOMY":["10090"]}}