iProXProteomicsHonglin WangMus Musculushttp://www.iprox.org/page/project.html?id=IPX0018390000Honglin.wang@sjtu.edu.cnShanghai Jiao Tong UniversityiProXfalseHSD17B4 interactome in mouse keratinocytes reveals association with splicing factors and RNA processingThis project aims to elucidate the molecular mechanism by which HSD17B4 regulates CYP7B1 expression in mouse keratinocytes (mKCs). To identify proteins that interact with HSD17B4, we performed co-immunoprecipitation (Co-IP) using an HSD17B4-specific antibody, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The raw mass spectrometry data were acquired and processed to identify HSD17B4-interacting proteins. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the HSD17B4 interactome revealed a significant overrepresentation of proteins involved in RNA splicing and RNA processing. These findings suggest that HSD17B4 may regulate CYP7B1 expression through interactions with splicing factors, pointing to a previously unrecognized nuclear function of HSD17B4 beyond its enzymatic activity. This project contains the MS data for the interacting protein of HSD17B4 we identified.Thu Jul 16 00:00:00 GMT+01:00 2026PXD08117010090