{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Lei Wang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018462000"],"submitter_email":["leiwang2021@gzhmu.edu.cn"],"submitter_affiliation":["Guangzhou Medical University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Identification of E3 Ubiquitin Ligase proteins with FOXJ1 and M2 Protein by Co-IP combined with mass spectrometry","description":"This project aims to systematically identify E3 ubiquitin ligases interacting with transcription factor FOXJ1 and M2 protein. Three experimental strategies were employed: (1) Co-transfection of FOXJ1-Flag and M2-myc in HEK293 cells, immunoprecipitated with IgG and myc antibodies; (2) Co-transfection of FOXJ1-Flag and M2-myc in HEK293T cells, immunoprecipitated with Flag antibody; (3) Overexpression of M2-myc in primary epithelial cells cultured under air-liquid interface differentiation, immunoprecipitated with IgG and myc antibodies.","dates":{"publication":"Sun Jul 19 00:00:00 GMT+01:00 2026"},"accession":"PXD081346","cross_references":{"TAXONOMY":["9606"]}}