{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Israel Ausin"],"species":["Arabidopsis Thaliana"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018491000"],"submitter_email":["israel.ausin@gmail.com"],"submitter_affiliation":["Northwest A&F University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"The absence of functional CAF-1 results in deficiencies in H3.3 levels at transcribed loci in Arabidopsis","description":"Eukaryotic organisms organize their genetic material in chromatin rather than naked DNA. Efficient chromatin assembly is essential for all DNA-templated processes, including replication, transcription, and DNA repair. Our research focused on two major histone H3 deposition complexes: Chromatin Assembly Factor (CAF-1), which deposits canonical H3 on naked DNA immediately after replication during the S-phase in yeast and metazoans, and the Histone Regulator A (HIRA) complex, which is thought to incorporate the H3.3 variant in a replication-independent manner. By combining biochemical, genetic, genomic, and proteomic data, we revealed that CAF-1 activity might be associated with the maintenance of the histone variant H3.3 in Arabidopsis chromatin. Moreover, this CAF-1's potential function in H3.3 homeostasis may be particularly significant in the transcribed regions of the genome.","dates":{"publication":"Tue Jul 21 00:00:00 GMT+01:00 2026"},"accession":"PXD081428","cross_references":{"TAXONOMY":["3702"]}}