{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Meng Zhao"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0018744000"],"submitter_email":["zhaomeng@tmu.edu.cn"],"submitter_affiliation":["Tianjin Medical University Cancer Institute &amp; Hospital"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Mass spectrometry-based identification of E3 ubiquitin ligases mediating MCT1 ubiquitination","description":"This project aims to identify potential E3 ubiquitin ligases regulating MCT1 (monocarboxylate transporter 1) in human cells. We performed immunoprecipitation of MCT1 followed by LC-MS/MS analysis using a Q-Exactive HF X mass spectrometer. Samples included control (con) and MCT1-pulldown (MCT1) groups, each with 7 biological replicates. Proteins were identified via Mascot search against the human SwissProt database (20,324 sequences) with FDR <1% at PSM level. A total of 398–1,201 proteins were identified per sample. The results will be used to screen for candidate E3 ligases interacting with MCT1.","dates":{"publication":"Mon Aug 03 00:00:00 GMT+01:00 2026"},"accession":"PXD082071","cross_references":{"TAXONOMY":["9606"]}}