iProXProteomicsMeng ZhaoHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0018744000zhaomeng@tmu.edu.cnTianjin Medical University Cancer Institute &amp; HospitaliProXfalseMass spectrometry-based identification of E3 ubiquitin ligases mediating MCT1 ubiquitinationThis project aims to identify potential E3 ubiquitin ligases regulating MCT1 (monocarboxylate transporter 1) in human cells. We performed immunoprecipitation of MCT1 followed by LC-MS/MS analysis using a Q-Exactive HF X mass spectrometer. Samples included control (con) and MCT1-pulldown (MCT1) groups, each with 7 biological replicates. Proteins were identified via Mascot search against the human SwissProt database (20,324 sequences) with FDR <1% at PSM level. A total of 398–1,201 proteins were identified per sample. The results will be used to screen for candidate E3 ligases interacting with MCT1.Mon Aug 03 00:00:00 GMT+01:00 2026PXD0820719606