iProXProteomicsShenhai GongMus Musculushttp://www.iprox.org/page/project.html?id=IPX0018788000gsh0526@smu.edu.cnSouthern Medical UniversityiProXfalseIntestinal β-galactosidase acts as a regulator of sepsis progressionThis project investigates the role of intestinal β-galactosidase in sepsis using 16S rRNA sequencing, metabolomic, and proteomic analyses. Septic patients showed reduced intestinal β-galactosidase activity associated with depletion of putative β-galactosidase-producing bacteria, including Blautia, Faecalibacterium, and Ruminococcus. Metabolomic profiling identified hesperetin as a key microbiota-associated metabolite, and proteomic analysis identified CDK13 as a critical downstream target regulating NLRP3 inflammasome priming and macrophage pyroptosis. Restoration of intestinal β-galactosidase promoted dietary hesperetin liberation and improved survival in septic mice.Wed Aug 05 00:00:00 GMT+01:00 2026PXD08225010090