iProXProteomicsTing DongHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0018855000tingdong2025@pumc.edu.cnInstitute of Medicinal Biotechnology (IMB), Chinese Academy of Medical Sciences & Peking Union Medical CollegeiProXfalseIdentification of FAK-interacting proteins by LC-MS/MS from silver-stained gel bandsFAK (focal adhesion kinase) is a key regulator of cell adhesion and signaling. To identify potential FAK-interacting proteins, we expressed FLAG-tagged FAK (FAK-FLAG) or empty vector (EV) control in HEK293T cells, followed by anti-FLAG affinity pull-down. The immunoprecipitated complexes were resolved by SDS-PAGE and visualized by silver staining. Specific protein bands enriched in the FAK-FLAG lane compared to the EV control were excised, subjected to in‑gel trypsin digestion, and analyzed by LC‑MS/MS. Protein identification was performed, revealing their potential association with FAK. This dataset provides the raw mass spectrometry data and identification results for these candidate interactors.Thu Aug 06 00:00:00 GMT+01:00 2026PXD0822609606