{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yang Chen"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0019159000"],"submitter_email":["chenyang1816185048@bjmu.edu.cn"],"submitter_affiliation":["The Center for Precision Medicine Multi-omics Research (CPMMR)"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"additional_accession":[]},"is_claimable":false,"name":"Stiffness Programs WDR44 Phosphorylation to Gate Cargo Sorting into Migrasomes","description":"Cells release extracellular vesicles (EVs) for intercellular communication, homeostasis, and microenvironment remodeling, yet how physiological matrix stiffness controls EV cargo sorting remains unclear. Here we show that stiffness programs a cargo-selective sorting pathway in migrasomes, where matrix stiffness couples Integrin β1–FAK–AKT to AKT-dependent phosphorylation of WDR44. Critically, phosphorylated WDR44 acts as a molecular gate that enables Rab11b-dependent trafficking of intraluminal nanovesicles via Myosin Va into migrasomes. Consistently, physiologically stiffness-programmed migrasomes enriched in CSF1 enhance macrophage-recruiting transport and accelerate wound closure in vitro. Together, these findings reveal a mechanistic framework for stiffness-regulated migrasome cargo sorting, and the tunable GA hydrogel platform (300–2,300 Pa) provides a scalable way to guide EV production under defined mechanical conditions.","dates":{"publication":"Mon Aug 24 00:00:00 BST 2026"},"accession":"PXD083164","cross_references":{"TAXONOMY":["10090"]}}