JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST000373/files/T110419_A05-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A06-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A13-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A12-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A04-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A01-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A03-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A11-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A10-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A02-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A28-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A27-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A26-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A25-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A24-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A23-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A22-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A39-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A08-KT.RAWhttps://storage.jpostdb.org/JPST000373/files/T110419_A09-KT.RAWprimaryOK200ProteomicsKeiichi I. NakayamaMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST000373Kyushu UniversityjPOSTFBX30, Fbxo30, FBXW6, SEL-10, hCdc4, Fbwd6, AGO, 1110001A17Rik, SEL10, CDC4, Cdc4, FBXO30, Fbw7-alpha, hAgo, Fbx30, Fbxw6, Fbw7., FBW6, Fbw7-gamma, FBW7Ubiquitin, close to, covalent modifier, Ubiquitylation, High Mobility Protein 20, single-organism developmental process, Peptidomics, Biocatalysts, postnatal development, postnatal growth, Human Ubiquitin, Ubiquitin-related 1, Synthetases, ATP Dependent Proteolysis Factor 1, growth and development, Ubiquitin carboxyl extension protein 80, CEP52, Human, near to, Ligase, APF-1, development, 40S ribosomal protein S27a, Enzyme, Ubiquitin A-52 residue ribosomal protein fusion product 1, 60S ribosomal protein L40, approaches, Ubiquitin-related 2, Associations, vicinity of, ubiquitin, Biocatalyst, Synthetase, Ubiquitin-related, HMG-20, Synthetases., ATP-Dependent Proteolysis Factor 1, protein tagging activity, growth, UbiqfalseDiPIUS_Fbxw7Although elucidation of enzyme–substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.Tue Mar 06 00:00:00 GMT 2018PXD00870510090