JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST000398/files/File%20relationship.xlsxhttps://storage.jpostdb.org/JPST000398/files/OSX_NAM_Prop.msfhttps://storage.jpostdb.org/JPST000398/files/Inclusion_list_OSX_NAM_Prop.msfhttps://storage.jpostdb.org/JPST000398/files/OSX_NaProp.msfhttps://storage.jpostdb.org/JPST000398/files/Inclusion_list_OSX_NAM_Prop.rawhttps://storage.jpostdb.org/JPST000398/files/OSX_NaProp.rawhttps://storage.jpostdb.org/JPST000398/files/OSX_NAM_Prop.rawhttps://storage.jpostdb.org/JPST000398/files/Inclusion_list_OSX_NAM_Prop.pdResulthttps://storage.jpostdb.org/JPST000398/files/OSX_NaProp.pdResulthttps://storage.jpostdb.org/JPST000398/files/OSX_NAM_Prop.pdResulthttps://storage.jpostdb.org/JPST000398/files/SDS-PAGE%20image%20for%20in-gel%20identification%20of%20OSX%20proK%20peptides.tifprimaryOK200ProteomicsNorie ArakiMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST000398Kumamoto UniversityjPOSTSP7/Osterix (OSX) is a master regulatory transcription factor that activates a variety of genes during differentiation of osteoblasts. However, the influence of post-translational modifications on the regulation of its transactivation activity is largely unknown. Here, we report that sirtuins, which are NAD(+)-dependent deacylases, regulate lysine deacylation-mediated transactivation of OSX. Germline Sirt7 knockout mice develop severe osteopenia characterized by decreased bone formation and an increase of osteoclasts. Similarly, osteoblast-specific Sirt7 knockout mice showed attenuated bone formation. Interaction of SIRT7 with OSX leads to the activation of transactivation by OSX without altering its protein expression. Deacylation of lysine (K) 368 in the C-terminal region of OSX by SIRT7 promote its N-terminal transactivation activity. In addition, SIRT7-mediated deacylation of K368 also facilitates depropionylation of OSX by SIRT1, thereby increasing OSX transactivation activity. In conclusion, our findings suggest that SIRT7 has a critical role in bone formation by regulating acylation of OSX.SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.Fukuda Masatoshi M, Yoshizawa Tatsuya T, Karim Md Fazlul MF, Sobuz Shihab U SU, Korogi Wataru W, Kobayasi Daiki D, Okanishi Hiroki H, Tasaki Masayoshi M, Ono Katsuhiko K, Sawa Tomohiro T, Sato Yoshifumi Y, Chirifu Mami M, Masuda Takeshi T, Nakamura Teruya T, Tanoue Hironori H, Nakashima Kazuhisa K, Kobashigawa Yoshihiro Y, Morioka Hiroshi H, Bober Eva E, Ohtsuki Sumio S, Yamagata Yuriko Y, Ando Yukio Y, Oike Yuichi Y, Araki Norie N, Takeda Shu S, Mizuta Hiroshi H, Yamagata Kazuya Kpolypeptide, peptide, Polypeptides, peptido, peptides, osterix, Peptid, peptidos, OSX, Polypeptide, Peptide., OI12, OI11Transcription Activation, T-cell leukemia, Materials, Activity, Lysine Hydrochloride, Laboratory, Mus domesticus, Ossification, AGL4, CG3066, CASP-14, Progress Reports, protein, PAE1, House Mouse, Social Controls, Physiological Ossification, Lysine Acetate, Genetic Induction, Roles, Summary Report, 6430578P22Rik, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, symptoms, Concepts, OGS1, 2, TRIM18, protein aggregate, present in fewer numbers in organism, Formal Social Controls, Summary Reports, ASF1A, L Lysine, Transcriptional, AA673258, Progress Report, K, osteogenesis, DmelCG11305, F10N7_150, epsilon-diaminocaproic acid, Swiss Mice, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Social, Progress, bone biosynthesis, Cementoclasts, decreased, Field Reports, LYS, Lysin, Sirtuin, Role Concepts, AGAMOUS-like 4, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, TRANSCRIPTION FACTOR, Physiological, RNA polymerase II distal enhancer sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific binding, screening, F4N21_13, D.mel5, CASP14, mouse, F4N21.13, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Generalized osteopenia, Osteopenia, anon-Ryu, Bone Disease, sir2l1, DmelCG3066, Sir2-like Proteins, Induction, Role Concept, Investigative Report, SIRT7, Mini-ICE, lysine, Role, Genetic Materials, Silent Mating Type Information Regulator 2 like Proteins, Osteoclastogeneses, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, Genetic Material, activation, region, Transactivation, F14P3.4, Mus musculus, Sir2alpha, Factors, Caspase-14 subunit p10, Endochondral Ossifications, mice, SIR2, Field, Swiss Mouse, Sir2, F14P3_4, Control, signs, Caspase-14 subunit p19, alpha, znfxy, Controls, proPO-AE, MICE, Transcription factor, domesticus, transcriptional transactivation, Bone Density, Report, rnf59., Sir2a, Material, Metabolic Bone Diseases, MP2, gbbb1, SIRTs, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, Cistron, Mouse, OSX, Osx, metal ion regulated sequence-specific DNA binding, Regulation, metal ion regulated proximal promoter sequence-specific DNA binding, Regulations, 3.4.22.-, XPRF, RNF59, Trans Activation, ZNFXY, osterix, MIDIN, xprf, Gene, osx, mini-ICE, SIR2L1, SEPALLATA 2, ANTI- SILENCING FUNCTION 1A, protein-containing complex, Transcription Factor, zinc ion regulated core promoter proximal region sequence-specific DNA binding, Investigative, reduced, House, Silent Mating Type Information Regulation 2 Homolog 1, subnumerary, SIR2L7, transactivation, Osteoclastogenesis, Gene Products, GBBB1, Mus musculus domesticus, Lysine, Low, Mice, Low Bone Mineral Density, bone formation, attenuated, Sirt1, SIRT, Transcription, CG11305, Genetic, Activation, Osteoclast, Swiss, Physiologic, sirtuin, dmSRT409, decreased number, midin, OI12, OI11, dSIRT7, RNA polymerase II proximal promoter sequence-specific DNA binding, Cementoclast, copper ion regulated proximal promoter sequence-specific DNA binding, site, Generalised osteopenia, Investigative Reports, SP7, sp7, Bone Formation, findings, Bone Loss, Metabolic Bone Disease, Formal Social Control, Sir2 like Proteins, Low Bone Densities, protein complex, BBBG1, AtSP7, Proteins, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, Endochondral, Endochondral Ossification, Cistrons, trim18, Concept, transcription factor activity, Low Bone Density, Osteopenias, native protein, Social Control, Mus, Acetate, Research Reports, Protein, RNA polymerase II transcription factor activity, sequence, Genetic Trans-Activation, rnf59, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, L-Lysine, Odontoclasts, KNOTTED1-like homeobox gene 5, Physiologic Ossification, C22, ogs1, FXY, Metabolic, Gene Activation, House Mice, Osteoblast, metal ion regulated core promoter proximal region sequence-specific binding, Odontoclast, copper ion regulated core promoter proximal region sequence-specific binding, F10N7.150, primary structure of sequence macromolecule, Ossifications, bbbg1, Laboratory Mice, Protein Gene Products, Silent Mating Type Information Regulator 2-like Proteins, Gene Proteins, OS, fxy, Reports, Osteopaenia, regulation, Summary, 6-diaminohexanoic acid, General activity, Enisyl, Laboratory Mouse, Severe, Field Report, Trans-ActivationLysine Hydrochloride, F4N21_13, AtSP7, D.mel5, Ossification, osterix, CG3066, F4N21.13, Endochondral, PAE1, Endochondral Ossification, ANTI- SILENCING FUNCTION 1A, Concept, anon-Ryu, Physiological Ossification, DmelCG3066, Lysine Acetate, Role Concept, Roles, SIRT7, Acetate, SIR2L7, 6430578P22Rik, Role, Osteoclastogenesis, lysine, Concepts, 2, Osteoclastogeneses, Lysine, bone formation, L-Lysine, Physiologic Ossification, L Lysine, ASF1A, C22, CG11305, osterix., osteogenesis, K, Endochondral Ossifications, Physiologic, DmelCG11305, epsilon-diaminocaproic acid, alpha, dmSRT409, proPO-AE, Ossifications, OI12, OI11, bone biosynthesis, dSIRT7, LYS, Lysin, Role Concepts, MP2, OSX, Osx, 6-diaminohexanoic acid, Enisyl, SP7, sp7, Bone Formation, PhysiologicalHEK293T, Hek 293T, Polypeptides, Human Embryonic Kidney 293T, peptido, 293-T, HEK-293T, peptides, 293tsA1609neo, osterix, Peptid, peptidos, Cell., HEK 293 T, Polypeptide, OSX, 293 T, Peptide, HEK-293-T, OI12, OI11, 293TfalseOsterix propionylated peptide identificationPropionylated peptides derived from over-expressed HA-tagged osterix in HEK293T cells were identified.Mon Nov 05 00:00:00 GMT 2018PXD0091471009030026585