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José Maschio"],"species":["Cellular Organisms"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST000457"],"submitter_affiliation":["UNISUL"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["Protein Gene Products, Gene Proteins, surface, soluble, external covering of organism, integumentary system, body surface, Protein, Acanthamoeba castellanius, proteomic analysis, Acanthamoeba castellani, Gene Products, Proteins, Gene, organism surface, Acanthamoeba, dermal system, Trophozoite., castellanius, integumentum commune"],"description_synonyms":["liquid chromatography tandem mass spectroscopy, Forms, Keratitides, Antemortem Diagnosis, determination, Infestations and Infections, Transient Blindness, Acquired Blindness, protein, Amauroses, Diagnosis, encephalomyelitis, Classifications, entry into host via enzymatic degradation of host cuticle, Polypeptides, Readability, encephalitis &/or myelitis, blindness, DSmurf, hierarchies, hierarchy, peptido, diseases, systematics, Myeloencephalitis, GRP1, Grp1, Bilateral Blindness, symptoms, SDS-PAGE, Impacts, diseases and disorders, Acanthamoeba, Environmental Impacts, Brain Inflammations, protein aggregate, animal, Encephalomyelitis NOS, integumentum commune, Myeloencephalitides, treatment, entry into host via a specialized structure during symbiotic interaction, human disease, me75, Polyacrylamide, cornea inflammation, LCMSMS, Man (Taxonomy), peptides, SDS-PAGE electrophoresis, pre-mortem, amoebae, PTPSTEP, Hysterical Blindness, Transient, free, D17Mit170, T1, Ameba, Encephalitis, Environmental Impact, infection of cornea, Inflammation, Smurf, l(2)k08110, disease management, Therapies, invasive growth, Homo sapiens disease, encephalitis, Infections and Infestations, CG4943, Diagnose, GPH, D-smurf, LC-MS2, Therapy, screening, external covering of organism, MYELOENCEPH, Brain Inflammation, Modern, Hysterical, LC-MS/MS, entry into cell of other organism during symbiotic interaction, Systematics, Legal Blindness, organism surface, Lack, Tl3, Tl2, Diagnoses, invasion of other organism, Acute onset, invasion into other organism, Taxonomies, organism, Postmortem, Screenings, soluble, Examinations and Diagnoses, Diseases, Encephalitis &/or myelitis, 3.1.3.48, NOS, entry into host through host barriers, Unilateral, Postmortem Diagnosis, clinical infection., Diagnoses and Examination, Encephalomyelitis, Postmortem Diagnoses, Acquired, absence, Legal, whole organism, death rate, Encephalitis/myelitis NOS, DmelCG4943, Sodium Dodecyl Sulfate-PAGE, Step, CG11628, Situation, Inflammatory Encephalomyelitis, Situational, signs, SDS polyacrylamide gel electrophoresis, Infection and Infestation, Striatum-enriched protein-tyrosine phosphatase, Treatments, living, disease, Parasite, Sudden, STEP, integumentary system, liquid chromatography-tandem mass spectroscopy, Corneal inflammation, Koerper, Situational Awareness, corneaitis, brain inflammation, Polypeptide, Situation Awareness, Sodium Dodecyl Sulfate-PAGEs, dermal system, humans, Brain, other disease, Awareness, multi-cellular organism, entry into cell of other organism involved in symbiotic interaction, GRP1/cytohesin 1, Visual Loss, taxonomy, entry into other organism during symbiotic interaction, dSmurf1, Encephalomyelitis NOS (disorder), central nervous system inflammation, Gene, ENCEPH INFLAMM, Gel Electrophoresis, protein-containing complex, SDS polyacrylamide gel electrophoresis of proteins, castellanius, Sudden Visual Losses, LC-MS-MS, Human, CG11633, cytohesin/GRP1, surface, Sodium Dodecyl Sulfate PAGE, Homo sapiens, Acanthamoeba castellanius, LC-MSMS, Acanthamoeba castellani, Polyacrylamide Gel Electrophoresis, Mass, Gene Products, disease or disorder, Screening, Low, Rasmussen's Syndrome, Antemortem, trophozoite stage, Man, Legal blindness, study, Taxonomy, Complete, Bilateral, entry into other organism involved in symbiotic interaction, absent from organism, l(2)SH2 0323, penetration into host via a specialized structure, Cyst, entry into host via enzymatic degradation of host anatomical structure, Diagnoses and Examinations, clinical infection, infective keratitis, non-neoplastic, Unilateral Blindness, Infestation and Infection, d-smurf, Neural-specific protein-tyrosine phosphatase, time of survival, disorder, Trophozoite, Situation Awarenesses, peptidos, species, corneal infection, keratitis caused by infection, Blindness, Inflammatory, SDS PAGE, inflammation of central nervous system, Antemortem Diagnoses, findings, cou, encephalitis and/or myelitis, body, protein complex, Complete Blindness, Proteins, disorders, whole body, stepk, medical condition, Dsmurf, Examination and Diagnoses, Peptide, Amaurosis, Impact, inflammation of cornea, Environmental, dSmurf, Situational Awarenesses, Sudden Visual Loss, Rasmussen Encephalitis, LC/MS/MS, Lr, survival, native protein, Mass Screenings, Smurf ubiquitin ligase, Protein, chemical analysis, proteomic analysis, Infection, keratitis, condition, Monocular Blindness, l(2)SH0323, Awarenesses, CYH1, INFLAMM ENCEPH, other organism invasion, body surface, sodium dodecyl sulphate–polyacrylamide gel electrophoresis, Understanding, DmelCG11628, Protein Gene Products, Gene Proteins, Rasmussen, entry into host cell via penetration peg, Therapeutic, Modern Man, Encephalitis/myelitis NOS (disorder), Bilateral Blindnesses, Environments, Bra, Monocular, liquid chromatography tandem mass spectrometry, Treatment, Peptid, penetration into host via a specialized structure during symbiotic interaction, assay, Rasmussen Syndrome, Encephalomyelitis (disorder)"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic analysis of soluble and surface-enriched proteins from Acanthamoeba castellanii trophozoites ","description":"Acanthamoeba castellanii is a free-living organism widely distributed in the environment that may cause disease. This protozoan exists in two forms, an infective trophozoite and a dormant cyst. The trophozoites are able to cause keratitis and granulomatous amoebic encephalitis in humans. Keratitis is an acute, sight threatening infection of cornea with potential to cause permanent blindness without prompt treatment. However, the lack of suspicion and the low awareness about these amoebae besides of the absence of commercially available immunodiagnostic tests may delay an accurate diagnosis. The identification of proteins with potential for use in immunodiagnosis may improve the parasite detection more quickly and specifically. The amoeba adhesion to the host cell is the primary step for infection but there is no full understanding of A. castellanii proteins relevant for host invasion or infection. In this study, an assessment of soluble and surface-enriched protein fractions expressed by A. castellanii trophozoites, based on complementary LC-MS/MS approach using peptides from SDS-PAGE excised bands, was performed. Our proteomic analysis allowed identification of a total of 503 proteins, of which 308 proteins were exclusively identified in the soluble fraction, 119 in surface-enriched fraction and 76 in both. In silico analysis of functional classification revealed several proteins involved in many biological mechanisms in A. castellanii, including pathogen survival and infection of mammalian hosts.","dates":{"publication":"Wed Jul 17 00:00:00 BST 2019"},"accession":"PXD010448","cross_references":{"TAXONOMY":["131567"]}}