{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["https://storage.jpostdb.org/JPST000467/files/Medusavirus%20whole%20proteins.raw"],"Mzid":["https://storage.jpostdb.org/JPST000467/files/Medusavirus%20whole%20proteins%20PD2.mzid"],"Mgf":["https://storage.jpostdb.org/JPST000467/files/Medusavirus%20whole%20proteins.mgf"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Masaharu Takemura"],"species":["Cellular Organisms"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST000467"],"submitter_affiliation":["Kyoto University"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"name_synonyms":["total expressed protein, Proteomes."],"description_synonyms":["BPBS, F10B6_15, Endopeptidase Inhibitor, Acide trichloracetique, Procedures, determination, Endopeptidase Inhibitors, Peptide Hydrolase Inhibitor, Gene, Protease, SDH, protein, abdominal muscles, protein-containing complex, F10B6.15, trichloro-, CG7826, Bca, SDS, method, Techniques, reduced, congenital, Ly57, Method, Protease Inhibitor, method used in an experiment, Gene Products, Studies, CG7835, CG42273, Inhibitors, AGM4, Min, Protease Antagonist, Peptide Hydrolase, tiny, Hydrolase Inhibitors, protein aggregate, trichloracetique, Technique, DmelCG42273, bca, treatment, Rubidium, beta-Trypsin, hypoplasia, min, 4733401P19Rik, mAPC, IBP, pk18, Antagonist, MBR, Trichloroacetic, lipomatosis of pancreas, EG:196F3.2, CG14783., AI047805, Study, Peptidohydrolase Inhibitor, Methodological Studies, SOLO DANCERS, Protease Antagonists, congenital lipomatosis of pancreas, disease management, Therapies, PTBR, Peptide Peptidohydrolase Inhibitors, Sodium Trichloroacetate, Shwachman-Bodian syndrome, Lyw-57, Bladder pain syndrome, 2-iodo-, BLNK-S, small, Therapy, DYRK1, Bonin Islands, BZRP, DBI, Peptidase Inhibitor, protein complex, Proteins, Dmel_CG7826, AI836084, Procedure, Peptide Peptidohydrolase, Peptide, PBS, PBR, SLP65, Ly-57, native protein, Temperatures, PKBS, Dm1, Protein, chemical analysis, Hydrolase Inhibitor, Trichloroacetate, Sodium, Rubidium Trichloroacetate, Dyrk1, eagle-Barrett syndrome, CG14784, Acetic acid, Dmel_CG7835, CG14783, Mnb, MNB, AU020952, Acid, absence of, SLP-65, Painful Bladder Syndrome, with urinary tract Abnormality and cryptorchidism, PRSS, Antagonists, BASH, underdeveloped, LY57, CC1, Shwachman-Diamond type metaphyseal dysplasia, Tripcellim, SWDS, Shwachman-Bodian-Diamond syndrome, Shwachman syndrome, Methodological, Peptidohydrolase Inhibitors, CGI-97, AW124434, mDRC, Methodological Study, beta Trypsin, Treatments, ME-IV, Protein Gene Products, plan specification, Gene Proteins, Trypure, Peptidase, Peptidase Inhibitors, Painful bladder syndrome, Antiprotease, Therapeutic, Acide, concentration, Pancreatic insufficiency and bone marrow dysfunction, Acetamide, Antiproteases, Treatment, Peptide Hydrolase Inhibitors, assay, Endopeptidase, Peptide Peptidohydrolase Inhibitor, DmelCG32810, Inhibitor, CG32810, Bladder Pain Syndrome"],"additional_accession":[]},"is_claimable":false,"name":"Proteome of Medusavirus","description":"Medusavirus particles was resuspended in PBS containing 0.5% SDS and protease inhibitor cocktail (Nacalai Tesque, Japan) and incubated for 1 h at 65°C. Samples were subjected to trichloroacetic acid (TCA) precipitation, followed by protein quantification using the BCA method. Proteins were reduced for 2 h at 37°C, and subsequently alkylated using iodoacetamide for 30 min at 20 to 25°C (room temperature), followed by treatment with trypsin for 20 h at 37°C. After desalination and concentration, the treated proteins were subjected to LC–MS/MS analysis using EASY-nLC 1200 (Thermo Fisher Scientific Inc., USA) and Q Exactive Plus (Thermo Fisher Scientific Inc., USA).","dates":{"publication":"Wed Jan 30 00:00:00 GMT 2019"},"accession":"PXD010830","cross_references":{"TAXONOMY":["131567"]}}