JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST000565/files/11C_131127121052.rawhttps://storage.jpostdb.org/JPST000565/files/5A_131113055702.rawhttps://storage.jpostdb.org/JPST000565/files/11.rawhttps://storage.jpostdb.org/JPST000565/files/5A.rawhttps://storage.jpostdb.org/JPST000565/files/9B.rawhttps://storage.jpostdb.org/JPST000565/files/9_131125033003.rawhttps://storage.jpostdb.org/JPST000565/files/11A_131126210917.rawhttps://storage.jpostdb.org/JPST000565/files/11B.rawhttps://storage.jpostdb.org/JPST000565/files/9A_131125110110.rawhttps://storage.jpostdb.org/JPST000565/files/5.rawhttps://storage.jpostdb.org/JPST000565/files/9C_131126060708.rawhttps://storage.jpostdb.org/JPST000565/files/9C.rawhttps://storage.jpostdb.org/JPST000565/files/5_131112222612.rawhttps://storage.jpostdb.org/JPST000565/files/9A.rawhttps://storage.jpostdb.org/JPST000565/files/9B_131125223541.rawhttps://storage.jpostdb.org/JPST000565/files/11A.rawhttps://storage.jpostdb.org/JPST000565/files/11_131126133808.rawhttps://storage.jpostdb.org/JPST000565/files/11B_131127044005.rawhttps://storage.jpostdb.org/JPST000565/files/9.rawhttps://storage.jpostdb.org/JPST000565/files/11C.rawprimaryOK200ProteomicsRosana De CastroHaloferax Volcanii Ds2https://repository.jpostdb.org/entry/JPST000565IIB-UNMdPjPOSTMembrane Tissue, membrane, transmembrane, Archaebacteria, Archaeobacteria, Metabacteria, determination, Tissues, archaea, membrane region, Tissue, number, integral to membrane, total expressed protein, Membrane, Monera, presence, Concept, Mendosicutes, Membrane Tissues, count in organism, Role Concept, Roles, Prokaryotae, prokaryote, integral component of membrane, Halobacterium volcanii., Role Concepts, chemical analysis, Role, region of membrane, whole membrane, Archaeon, Concepts, prokaryotes, Procaryotae, assay, Archaebiota, Proteomes, Archebacteria, ProkaryotaIntegral Membrane Proteins, d230, 11', Peptidomics, Ass-1, Surface Proteins, Gene, LonHS, dTAFII250, Membrane-Associated Proteins, PIM1, Integral, Ximpact, EfW1, Downregulation, supernumerary, protrusion, Mendosicutes, 11, Mutations, 12, dmTAF[[II]]230, Membrane Tissues, DmelCG8798, resilient, Lon, LON, dmTAF1, Taf230, integral component of membrane, Membrane-Associated Protein, AA408052, tough, Gene Products, fold, Extreme Environment, 7, 8, 15-cis-phytoene, TAF250, imprinted and ancient gene protein, Cell Membrane, study, strong, increased, Taf200, dTAF[[II]]250, TFIID TAF250, reference sample, Metabacteria, lon, all-trans-phytoene, cel, Tissues, cell, Surface, membrane region, Tissue, Lag1., Taf1p, Monera, ASS, dTAF250, Prokaryotae, (all-E)-, psi-carotene, region of membrane, CG3576, Surface Protein, Membrane Associated Proteins, Procaryotae, associated, Archaebiota, TAF, anon-WO0118547.350, Membrane Protein, Controlled, Membrane Tissue, membrane, Controlling, Archaebacteria, Archaeobacteria, dTAF[[II]]230, TAF[[II]]250, (15-cis)-isomer, anatomical protrusion, Proteins, TAF200, integral to membrane, total expressed protein, Cell Surface, l(3)84Ab, CG8798, Cell Surface Protein, function, BG:DS00004.13, TAFII-250, TAF250/230, impact-a, Cell, Down Regulation, predicted, dTAF230, DLag1, DLAG1, TAFII250, Prss15, LONP, Down-Regulation, 12'-octahydro-psi, PRSS15, Halobacterium volcanii, Receptor Down-Regulation, soluble, trans-phytoene, Down-Regulation (Physiology), p230, Protein, whole membrane, Membrane Proteins, (cis)-isomer, TAF[[II]]250/230, imprinted and ancient gene protein homolog, IMPACT, TFIID, prokaryotes, Environment, l(1)G0061, Archebacteria, Cell Surface Proteins, phytoene, Membrane Associated Protein, Taf[[II]]250, Cell Membrane Protein, transmembrane, DmelCG3576, TAF[[II]]230, increased number, archaea, Membrane-Associated, 7', Dlag1, Cell Membrane Proteins, TAF[II]250, Membrane, 1200017E13Rik, CG17603, Lag1, TAF[[II]], Protein Gene Products, l(1)G0365, Gene Proteins, present in greater numbers in organism, Integral Membrane Protein, DmelCG17603, Extreme, Taf250, spine, prokaryote, SR3-5, lag1, l(1)G0489, 8', Archaeon, Environments, hLON, Receptor, E430016J11Rik, CG15898, Proteomes, RWDD5, Prokaryota, accessory, TAF230, Integral Membrane, TAF1falseGlobal role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcaniiThe membrane-associated LonB protease is essential for viability in Haloferax volcanii, however, the cellular processes affected by this protease in archaea are unknown. In this study, the impact of a lon conditional mutation (down-regulation) on H. volcanii physiology was examined by comparing proteomes of parental and mutant cells using shotgun proteomics. A total of 1778 proteins were identified (44% of H. volcanii predicted proteome) and 142 changed significantly in amount (≥2 fold). Of these, 66 were augmented in response to Lon deficiency suggesting they could be Lon substrates. The “Lon subproteome” included soluble and predicted membrane proteins expected to participate in diverse cellular processes. The dramatic stabilization of phytoene synthase (57 fold) in concert with overpigmentation of lon mutant cells suggests that Lon controls carotenogenesis in H. volcanii. Several hypothetical proteins, which may reveal novel functions and/or be involved in adaptation to extreme environments, were notably increased (300 fold). This study, which represents the first proteome examination of a Lon deficient archaeal cell, shows that Lon has a strong impact on H. volcanii physiology evidencing the cellular processes controlled by this protease in Archaea. Additionally, this work provides a platform for the discovery of novel targets of Lon proteases.Wed Mar 11 00:00:00 GMT 2020PXD013046309800