{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Xlsx":["https://storage.jpostdb.org/JPST000592/files/140310_Dnm1WT_Scaffold.xlsx"],"Csv":["https://storage.jpostdb.org/JPST000592/files/140227_PeotideIDs_Dnm1WT.csv"],"Raw":["https://storage.jpostdb.org/JPST000592/files/140224-dnmwt.raw"],"Other":["https://storage.jpostdb.org/JPST000592/files/140310_Dnm1.sf3"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Sachihiro Matsunaga"],"species":["Cyanidioschyzon Merolae"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST000592"],"submitter_affiliation":["Tokyo University of Science"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["The mitochondrion is an organelle that was derived from an endosymbiosis. Although regulation of mitochondrial growth by the host cell is necessary for the maintenance of mitochondria, it is unclear how this regulatory mechanism was acquired. To address this, we studied the primitive unicellular red alga <i>Cyanidioschyzon merolae</i>, which has the simplest eukaryotic genome and a single mitochondrion. Here we show that the <i>C. merolae</i> Aurora kinase ortholog CmAUR regulates mitochondrial division through phosphorylation of mitochondrial division ring components. One of the components, the Drp1 ortholog CmDnm1, has at least four sites phosphorylated by CmAUR. Depletion of the phosphorylation site conserved among eukaryotes induced defects such as mitochondrial distribution on one side of the cell. Taken together with the observation that human Aurora kinase phosphorylates Drp1 in vitro, we suggest that the phosphoregulation is conserved from the simplest eukaryotes to mammals, and was acquired at the primitive stage of endosymbiosis."],"pubmed_title":["<i>Cyanidioschyzon merolae</i> aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division."],"pubmed_authors":["Kato Shoichi S, Okamura Erika E, Matsunaga Tomoko M TM, Nakayama Minami M, Kawanishi Yuki Y, Ichinose Takako T, Iwane Atsuko H AH, Sakamoto Takuya T, Imoto Yuuta Y, Ohnuma Mio M, Nomura Yuko Y, Nakagami Hirofumi H, Kuroiwa Haruko H, Kuroiwa Tsuneyoshi T, Matsunaga Sachihiro S"],"name_synonyms":["Phosphorylations, phosphorylation."],"pubmed_title_synonyms":["Aurora, Kinase, mitochondrial proliferation., Aurora Kinase"],"pubmed_abstract_synonyms":["Dnmlp1, Regulations, positioning of mitochondria, d230, human being, P62, postnatal development, Addresses, Aurora, DPYSL1, Eukaryotes, dTAFII250, growth and development, RING, Organelle, sci, Mitochondrial Contractions, mitochondrial, EfW1, dRing, phosphorylation, Social Controls, Human, dring, python, dmTAF[[II]]230, Homo sapiens, dmTAF1, Taf230, Mitochondrion, HOW, How, dRING, mammals, Kinase, HDYNIV, Formal Social Controls, Man, l(3)j5D5, Ding, TAF250, 24B, DRP-1, hYAK3-2, Taf200, dTAF[[II]]250, l(3)s2612, Man (Taxonomy), Dlp1, DLP1, TFIID TAF250, Genomes, cel, cell, Contraction, stru, Drp-1, Taf1p, l(3)S053606, dRING1, Eucarya, Maintenances, T16H5_60, CG10293, eucaryotes, Social, l(3)j5B5, DYMPLE, dTAF250, DmelCG10293, RED, Red, drp-1, site, stage, Ring, CG5595, CG3210, TAF, ULIP-3, DYRK5, DRP, Drp, 0904/17, dTAF[[II]]230, TAF[[II]]250, Dring, Eukaryote, mitochondrial proliferation, CRMP-1, Formal Social Control, clone 2.39, Modern, DRING, Mammal, SMAP-3, Phosphorylations, TAF200, l(3)84Ab, Eukaryotas, BG:DS00004.13, qkr, TAFII-250, Dnm1/Drp1, TAF250/230, l(3)S090417, not genetically inherited, Cell, HMG-CoAR, CSA2, eukaryotes, dTAF230, development, Contractions, dRing1, RING1, TAFII250, ATP synthase D chain, Social Control, SZ1, T16H5.60, p230, KH93F, TAF[[II]]250/230, l(2)22Fd, TFIID, Ring/Sce, l(2)22Fc, DVLP, region, EMPF, who, DmelCG3210, Taf[[II]]250, DmelCG5595, Mitochondrial, TAF[[II]]230, growth pattern, non-developmental growth, Aurora Kinase, Rnf2, Drp1, DRP1, frat, postnatal growth, Control, Eukarya, TAF[II]250, Who/How, whole genome, Controls, 6330417M19Rik, CG17603, TAF[[II]], REDK, l(2)ND3, human, l(2)ND2, Mitochondrial Contraction, drp1, Eukaryotae, mitochondria, DmelCG17603, Taf250, SR3-5, Modern Man, Sce/dRing, qkr[93F], DMDL, anon-EST:Liang-2.39, Mammalia, regulation, SCE, Eucaryotae, Regulation, growth, developmental stage., TAF230, AI450666, TAF1"],"description_synonyms":["Dnm, DNM, proto-oncogene c-Kit, nebb, 38B.12, KIF14, CG10718, 38B.10, protein complex, DmKlp38B, Proteins, Aurora, SCF receptor activity, Gene, Tr-kit, Ftfl, protein, Xkl-1, protein-containing complex, tio, Peptide, DmelCG10718, KLP-38B, Polypeptides, PBT, l(2)03552, KL receptor activity, Gsfsco1, native protein, peptido, scfr, KIT ligand receptor activity, Protein, sl(2)ry, Klp38B, Gene Products, SCO5, kl1-A, SCO1, Kinase, KIT, protein aggregate, Gsfsow3, l(2)k00802, SCFR, Gsfsco5, SOW3, XKrk1, KLP38B, tyrosine-protein kinase Kit, pbt, c-KIT, Fdc, l(2)k07614, peptides, Aurora Kinase, klp38B, AI838169, W, kit, mKIAA4093, CD117, Protein Gene Products, Gene Proteins, c-kit, Klp38, C-Kit, Neb, Dm0332, Ssm, xkl-1, Bs, Peptid, peptidos, Mot, Polypeptide, KLP 38B, sl(2)ry3, Sl, Peptide., krk1"],"additional_accession":[]},"is_claimable":false,"name":"CmDnm1, in vitro phosphorylation by CmAUR","description":"Recombinant Cyanidioschyzon Dnm1 protein (CmDnm1) was incubated with recombinant Cyanidioschyzon Aurora kinase (CmAUR). Proteins were tryptic digested and the phosphorylated peptides were concentrated with Titansphere Phos -TiO Kit. Finally, the enriched peptides were analyzed by LC-MS.","dates":{"publication":"Fri Dec 27 00:00:00 GMT 2019"},"accession":"PXD013717","cross_references":{"TAXONOMY":["45157"],"pubmed":["31886415"]}}