JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST000613/files/TN9.rawhttps://storage.jpostdb.org/JPST000613/files/TN9.mgfhttps://storage.jpostdb.org/JPST000613/files/Mascot%20Search%20Results%20Protein%20View.htmprimaryOK200ProteomicsNobutaka HirokawaMus Musculus (mouse)https://repository.jpostdb.org/entry/JPST000613Gunma UniversityjPOSTThe axon initial segment (AIS) is a compartment that serves as a molecular barrier to achieve axon-dendrite differentiation. Distribution of specific proteins during early neuronal development has been proposed to be critical for AIS construction. However, it remains unknown how these proteins are specifically targeted to the proximal axon within this limited time period. Here, we reveal spatiotemporal regulation driven by the microtubule (MT)-based motor KIF3A/B/KAP3 that transports TRIM46, influenced by a specific MARK2 phosphorylation cascade. In the proximal part of the future axon under low MARK2 activity, the KIF3/KAP3 motor recognizes TRIM46 as cargo and transports it to the future AIS. In contrast, in the somatodendritic area under high MARK2 activity, KAP3 phosphorylated at serine 60 by MARK2 cannot bind with TRIM46 and be transported. This spatiotemporal regulation between KIF3/KAP3 and TRIM46 under specific MARK2 activity underlies the specific transport needed for axonal differentiation.The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.Ichinose Sotaro S, Ogawa Tadayuki T, Jiang Xuguang X, Hirokawa Nobutaka Nmeasuring, Transphosphorylases, AI385631, TRIFIC, determination, scientific observation, Kinases, GENEY, chemical analysis., Kinase, assay, ATP Phosphotransferases, Transphosphorylase, Phosphotransferase, Phosphotransferases, ATPRegulations, SBMA, Par1b, single-organism developmental process, Activity, Effects, klp64D, p51, supply, P62, postnatal development, Smg-GDS, Par-1b, Gene, growth and development, dJ190I16.1, sci, neurotubule, TFM, cargo, NBP, phosphorylation, Long Term, Social Controls, 2-Amino-3-hydroxypropionic acid, DHTR, testicular feminization syndrome (formerly), solute:solute exchange, CG11759, KLP4, Klp4, mKIAA4207, Axon Initial Segments, AIS, p63, B(p51B), SMAP, TP73L, Gene Products, HOW, How, DmelCG11759, Feminisation - testicular, DmF2, Low, DmKlp64D, androgen receptor deficiency, Fs(3)Hor, Effect, KET, Formal Social Controls, microtubulus, l(3)j5D5, 111-11-71, Axonal Trigger Zones, lod, 24B, Kif3A, DmelCG2684, me75, KLP64Ddm, OFC8, l(3)s2612, Longterm, distribution, B(p51A), Axonal Trigger Zone, p73H, stru, KIF3A, l(3)S053606, Long-Term, KD, NTef2, CG10293, D17Mit170, par-1, T1, Social, l(3)j5B5, PAR-1, emk-1, FLA10, 2-amino-3-hydroxypropanoic acid, Axon, SHFM4, Dendrite, DmelCG10293, Par-1, Long-Term Effect, microtubuli, EMK-1, FLA3, Long-Term Effects, DmKap, DmKAP, Axon Hillocks, 0904/17, par1, initial segment, single-organism transport., cou, TRIFIC, Formal Social Control, clone 2.39, Proteins, Longterm Effect, Phosphorylations, AW124694, KLP64D, qkr, KAP3, Tl3, Axonal, l(3)S090417, Tl2, Fs(3)Sz11, development, Emk, Menstruation, Serin, Lr, Kif3, Period, AU024026, 3-Hydroxyalanine, Axon Hillock, Social Control, SZ1, kap3, KH93F, Protein, Long Term Effects, p73L, TP53L, supply and distribution, p53CP, xklp3A, dKAP3, Microtubule, KAP, Kap, who, Kns3, AI385631, DHTR deficiency, Smg GDS-associated protein, EMK1, EEC3, TP53CP, postnatal growth, Control, Who/How, 111-11-86, Controls, Lds, RHS, early, Longterm Effects, KAP-3, Protein Gene Products, KAP-1, emk1, Gene Proteins, HYSP1, CG10642, AR deficiency, Kifl, par1b, Horka, dihydrotestosterone receptor deficiency, p40, GENEY, CG2684, Bra, NR3C4, Fs(3)Horka, qkr[93F], small molecule transport, LMS, anon-EST:Liang-2.39, KLP-20, L Serine, regulation, SMAX1, DmelCG10642, L-Serine, Trigger Zone, growth, Regulation, General activity, HUMARADmKap, DmKAP, Axon Hillocks, par1, initial segment, Par1b, TRIFIC, Smg-GDS, Par-1b, AW124694, dJ190I16.1, KAP3, Axonal, Emk, solute:solute exchange, CG11759, Kif3, mKIAA4207, AU024026, Axon Hillock, Axon Initial Segments, single-organism transport, kap3, SMAP, DmelCG11759, dKAP3, KAP, Kap, 111-11-71, Axonal Trigger Zones, Kns3, AI385631, Smg GDS-associated protein, EMK1, Axonal Trigger Zone, 111-11-86, par-1, KAP-3, KAP-1, emk1, PAR-1, emk-1, Kifl, signalling process, par1b, GENEY, Par-1, small molecule transport, EMK-1, FLA3, Trigger Zone, single organism signaling.Transphosphorylases, AI385631, DmelCG8203, cdk5, TRIFIC, determination, PSSALRE, GENEY, chemical analysis, AW048668, CDK5, assay, Kinase, CG8203, Kinases., ATP Phosphotransferases, Transphosphorylase, Crk6, Phosphotransferase, DmCdk5, Phosphotransferases, ATPfalseTRIM46 Kinase AssayPhosphoproteomic analysis of recombinant TRIM46 reacted with CDK5 kinase in vitro.Thu Aug 29 00:00:00 BST 2019PXD0141091009031461655