JPOST Repositoryapplication/xmlhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP389.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP378.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP387.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP385.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP380.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP394.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP381.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP396.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP391.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP377.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP383.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP388.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP379.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP386.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP392.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP393.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP382.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP384.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP390.rawhttps://storage.jpostdb.org/JPST000877/files/200224_C20_009_QHF1_Standard_Rev02_%2320_015_PAP395.rawprimaryOK200ProteomicsUte ArmbrusterArabidopsis Thaliana (mouse-ear Cress)https://repository.jpostdb.org/entry/JPST000877University of Münster - WWU - Institute for Biology and Biotechnology of PlantsjPOSTDuring photosynthesis, energy is transiently stored as an electrochemical proton gradient across the thylakoid membrane. The resulting proton motive force (pmf) is composed of a membrane potential (ΔΨ) and a proton concentration gradient (ΔpH) and powers the synthesis of ATP. Light energy availability for photosynthesis can change very rapidly and frequently in nature. Thylakoid ion transport proteins buffer the effects that light fluctuations have on photosynthesis by adjusting pmf and its composition. Ion channel activities dissipate ΔΨ, thereby reducing charge recombinations within photosystem II. The dissipation of ΔΨ allows for increased accumulation of protons in the thylakoid lumen, generating the signal that activates feedback downregulation of photosynthesis. Proton export from the lumen via the thylakoid K+ exchange antiporter 3 (KEA3), instead, decreases the ΔpH fraction of the pmf and thereby reduces the regulatory feedback signal. Here, we reveal that the Arabidopsis (Arabidopsis thaliana) KEA3 protein homo-dimerizes via its C-terminal domain. This C-terminus has a regulatory function, which responds to light intensity transients. Plants carrying a C-terminus-less KEA3 variant show reduced feed-back downregulation of photosynthesis and suffer from increased photosystem damage under long-term high light stress. However, during photosynthetic induction in high light, KEA3 deregulation leads to an increase in carbon fixation rates. Together, the data reveal a trade-off between long-term photoprotection and a short-term boost in carbon fixation rates, which is under the control of the KEA3 C-terminus.Functional characterization of proton antiport regulation in the thylakoid membrane.Uflewski Michał M, Mielke Sarah S, Correa Galvis Viviana V, von Bismarck Thekla T, Chen Xiaoheng X, Tietz Enrico E, Ruß Jeremy J, Luzarowski Marcin M, Sokolowska Ewelina E, Skirycz Aleksandra A, Eirich Jürgen J, Finkemeier Iris I, Schöttler Mark Aurel MA, Armbruster Ute Uefflux.Water, Photosynthesis Water-Splitting Enzyme, Striadyne, ion, Adenosine Triphosphate, Antiport, protein fingerprinting, Membrane Potential, A., Facilitated Ion Transport, Visible Light, protein, Ion Exchange, composed of, Arabis thaliana, Photosynthetic Carbon Fixation, Cardaminopsis, MAGE-E1 antigen, ter, Arabidopsis thalianas, Flux, Toxicity and JNK inducer, A. thalianas, Hominids, Exchange, PRB1M, Transmembrane Potential Differences, PRB1L, protein aggregate, Carbon Fixation, prevention and control, multicellular organismal biosynthetic process, increased, single-organism biosynthetic process, Fixations, Chromium Adenosine Triphosphate, reference sample, availability, Differences, Carbon Fixations, Photosystem II Reaction Center Complex D2 Protein, long, Mouse-ear Cress, Evolving Enzyme, hypoplasia, composition, prevention., plants, Ion Cotransport, Calvin-Benson-Bassham, Mouse-ear, Delta Psi, preventive measures, Oxygen, TNFSF14, ATPsyn b, Cotransport, CrATP, HCA1, Hominin, Bsg75C, Cr(H2O)4 ATP, Carbon, Transmembrane Potentials, Carbon Fluxs, Calvin, Fixation, Arabidopses, Light-Induced D1 Protein, ATP, Hominid, Chromium Ammonium Salt, Intracellular Ion Exchange, ATPsyn-&bgr, Oxygen Evolving Enzyme, reaction centre, Facilitated, preventive therapy, Radiation, ATPsyn-b, Difference, Resting Potential, UNQ391/PRO726, land plants, TAJ-alpha, Carbon Cycles, Light, A. thaliana, Calvin Benson Cycle, shortened, PRCP II D2 Protein, LIGHT, Ionen, Term, DAMAGE, Hepatocellular carcinoma-associated protein 1, Manganese Salt, Magnesium Salt, Arabidopsis thaliana (thale cress), Hydrogen Ions, Hydrogen Ion, CG11154, Visible Radiations, Potential Differences, Visible Radiation, Oxidase, HVEML, content, TROY, Intracellular Ion, Plant, Calvin Benson Bassham Cycle, CaATP, Arabidopsis, higher plants, Resting Potentials, ion transport, MgATP, Grana, Light Induced D1 Protein, Resting Membrane Potentials, RCII-D1 Protein, Feedbacks, short, mMage-e1, Intracellular, Symport, humans, accessory, Passive, Active Ion Transport, Transmembrane, Photosystem II Reaction Center Complex D1 Protein, Magnesium Adenosine Triphosphate, Potential, ATP-MgCl2, Gene, Reductive Pentose Phosphate Cycle, Passive Ion Transport, Potential Difference, IB, protein-containing complex, Hominini, reaction center, supernumerary, Adenylpyrophosphate, Ly113, Buffer, Apes, reduced, Homo, ATP-synbeta, Photosynthesis Dark Reactions, Cycle, Gene Products, thalianas, Carrying, tiny, Transport, Water Oxidase, Cycles, Transmembrane Electrical Potential Difference, DmelCG11154, Cresses, Transmembrane Potential, iones, carbon dioxide fixation, beta-ATPase, ATP MgCl2, stubby, Pongidae, Proton, Visible, AI847422, ions, Active Ion, Hominins, thale-cress, Enzyme, DmelCG4216, Chromium Salt, ATPasebeta, MnATP, ATPase beta, Calvin-Benson-Bassham Cycle, Uniport, Cress, Carbon Flux, TR2, Thylakoid Membrane, Mouse ear, Dark Reactions of Photosynthesis, ATPB, TAJ, Controlled, small, TRADE, Controlling, lumen, Fluxs, Photosystem II, Arbisopsis thaliana, Thylakoid, protein complex, Photosynthetic, synthesize, space, Proteins, Arabidopsis thaliana, Alpha-dystrobrevin-associated MAGE Protein, Photosynthesis Dark Reaction, Magnesium Chloride, inhibition of photosynthesis, function, compositionality, Adenosine 5'-(tetrahydrogen triphosphate), CD258, buffer, Homininus, ATP-syn-B, Atriphos, ML-1, native protein, Ions, down regulation of photosynthesis, Water Splitting Enzyme of Photosynthesis, Protein, Thylakoid Membranes, feed, Active, D1 Photosystem II Protein, Calvin-Benson Cycle, Oxygen Evolving, anatomical spaces, Photosystem II Reaction Center, Radiations, thale cress, mouse-ear cress, Membranes, lumen space, Hydrogen, Manganese Adenosine Triphosphate, underdeveloped, RGD1560259, HVEM-L, Resting, increased number, prophylaxis, Photoradiation, CG4216, thaliana, photosynthetic membrane, Membrane, Ion Transport, Mouse-ear Cresses, LTg, Protein Gene Products, Gene Proteins, present in greater numbers in organism, Transmembrane Potential Difference, Photoradiations, PMF, down-regulation of photosynthesis, Ion, Calvin Cycle, concentration, control, ATPIB, structure, downregulation of photosynthesis, Potentials, Resting Membrane Potential, Membrane Potentials, PMS, Ape, Water-Splitting Enzyme of Photosynthesis, Calvin-Benson, Chloroplast Reaction Center Protein D1, PM, Calcium SaltIntracellular Ion Exchange, Regulations, Passive, Active Ion Transport, Facilitated, Antiport, Formal Social Control, Thylakoid, exchange transporter activity, Facilitated Ion Transport, Passive Ion Transport, Ion Exchange, porter, Social Controls, countertransporter activity, solute:solute exchange, Thylakoid., Ions, Social Control, Exchange, Thylakoid Membranes, Active, exchanger, Hydrogen Ions, Formal Social Controls, Hydrogen Ion, Transport, Membranes, Hydrogen, antiport, Intracellular Ion, Control, Ion Cotransport, Proton, Controls, Membrane, Ion Transport, ion antiporter activity, solute:solute antiporter activity, Active Ion, Social, Cotransport, Ion, Uniport, regulation, Grana, Thylakoid Membrane, Regulation, Intracellular, SymportMembranes, Separation, Genogroups, Divorced, BP2, Genogroup, ATREC8, Thylakoid, MOP10_3, REC8, Separations, Thylakoid Membranes, DIF1, INFERTILE 1, Grana, Thylakoid Membrane, Divorces, Genotypes., SYNAPTIC 1, Membrane, Separated, DETERMINATE, MOP10.3falseInteraction partners of the K(+) efflux antiporter 3Thylakoids were isolated, solubilized and separated by BN-PAGE in order to determinate complex compositions in different genotypesThu Apr 01 00:00:00 BST 2021PXD019865370233742682