{"database":"JPOST Repository","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Raw":["https://storage.jpostdb.org/JPST000964/files/yk-HD38.raw","https://storage.jpostdb.org/JPST000964/files/yk-271.raw","https://storage.jpostdb.org/JPST000964/files/yk-M1.raw","https://storage.jpostdb.org/JPST000964/files/yk-B44.raw","https://storage.jpostdb.org/JPST000964/files/yk-241.raw","https://storage.jpostdb.org/JPST000964/files/yk-182.raw","https://storage.jpostdb.org/JPST000964/files/yk-184.raw","https://storage.jpostdb.org/JPST000964/files/yk-40.raw","https://storage.jpostdb.org/JPST000964/files/yk-B46.raw","https://storage.jpostdb.org/JPST000964/files/yk-B34.raw","https://storage.jpostdb.org/JPST000964/files/yk-270.raw","https://storage.jpostdb.org/JPST000964/files/yk-218.raw","https://storage.jpostdb.org/JPST000964/files/yk-248.raw","https://storage.jpostdb.org/JPST000964/files/yk-216.raw","https://storage.jpostdb.org/JPST000964/files/yk-41.raw","https://storage.jpostdb.org/JPST000964/files/yk-HD42.raw","https://storage.jpostdb.org/JPST000964/files/yk-265.raw","https://storage.jpostdb.org/JPST000964/files/yk-249.raw","https://storage.jpostdb.org/JPST000964/files/yk-238.raw","https://storage.jpostdb.org/JPST000964/files/yk-263.raw"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yoshie Kametani"],"species":["Homo Sapiens (human)","Mus Musculus (mouse)"],"full_dataset_link":["https://repository.jpostdb.org/entry/JPST000964"],"submitter_affiliation":["Tokyo Metropolitan Institute of Medical Science"],"sample_protocol":[""],"repository":["jPOST"],"data_protocol":[""],"pubmed_abstract":["The status of humoral immunity of cancer patients is not clear compared to cellular immunity because the ability of specific antibody production is difficult to analyze in vitro. We previously developed a humanized mouse model to evaluate antigen-specific antibody production by transplanting human peripheral blood mononuclear cells (PBMCs) into NOG-hIL-4-Tg mice (hu-PBL hIL-4 NOG). In this study, these mice were transplanted with PBMCs derived from breast cancer patients (BC) and immunized with a human epidermal growth factor receptor 2 (HER2) peptide, CH401MAP, to analyze humoral immunity of BCs. The hu-PBL hIL-4 NOG mice recapitulated immune environment of BCs as the ratio of CD8+/CD4+T cells was lower and that of PD-1 + T cells was higher compared to healthy donors (HDs). Diverse clusters were detected in BC-mouse (BC-M) plasma components involving immunoglobulins and complements unlike HD-M, and there was a significant diversity in CH401MAP-specific IgG titers in BC-M. The number of B cell clones producing high CH401MAP-specific IgG was not increased by immunization in BC-M unlike HD-M. These results demonstrated that the humoral immunity of BCs appeared as diverse phenotypes different from HDs in hu-PBL hIL-4 NOG mice, which may provide important information for the study of personalized medicine."],"pubmed_title":["HER2-antigen-specific humoral immune response in breast cancer lymphocytes transplanted in hu-PBL hIL-4 NOG mice."],"pubmed_authors":["Ohno Yusuke Y, Ohshima Shino S, Miyamoto Asuka A, Kametani Fuyuki F, Ito Ryoji R, Tsuda Banri B, Kasama Yukie Y, Nakada Shunsuke S, Kashiwagi Hirofumi H, Seki Toshiro T, Yasuda Atsushi A, Ando Kiyoshi K, Ito Mamoru M, Tokuda Yutaka Y, Kametani Yoshie Y"],"name_synonyms":["Mus musculus, human being, Man (Taxonomy), Peptidomics, Laboratory, Protein., Swiss, protein complex, mice, Blood, Modern, Mus domesticus, Proteins, mouse, Swiss Mouse, Gene, House Mice, Swiss Mice, protein, Blood Serum, Serum, protein-containing complex, House Mouse, human, domesticus, Laboratory Mice, Human, Protein Gene Products, Gene Proteins, native protein, Homo sapiens, House, Mus, Modern Man, Protein, Gene Products, Mus musculus domesticus, Mouse, house mouse, protein aggregate, Mice, Man, Laboratory Mouse, Serums"],"pubmed_abstract_synonyms":["Erbb-2, IgG Antibody, malignant tumor of the breast, B Cells, T-cell surface antigen T4/Leu-3, Peripheral Blood Mononuclear Cells, Laboratory, Bursa-Dependent Lymphocytes, Blood, Globulin, Mus domesticus, FLNMS, Immune Globulin, B cell, mammary neoplasm, Hs.6719, Human Mammary Neoplasms, CASP-14, nog1, Tumor, 2-oxo-3-azaglutaric acid, Sensitization, House Mouse, BCS1, CD340, peptide, Polypeptides, Speciality, L3T4, Kiaa3023, peptido, \"neoplasm of breast (disorder)\" EXACT [SNOMEDCT_2005_07_31:126926005], Gamma Globulin, Specialities, Antigen, Ly-4, Impacts, BCS, Peripheral Blood Mononuclear Cell, cancer of the breast, Immunological Sensitization, Environmental Impacts, antibody production, HER2delta16 protein, HER-2 protein, BC, Mammary Neoplasms, malignant neoplasm of breast, Fresh Frozen Plasmas, Fresh Frozen, increased, Immune Processes, Immune Responses, Insurance Medicine, Man (Taxonomy), peptides, Sensitizations, SYM1, DmelCG30147, Tissue, Human Mammary Carcinomas, BCs, Swiss Mice, SYNS1, epidermal growth factor receptor 2, Environmental Impact, CG13435, Mononuclear, Immune, B lymphocyte, Mononuclear Leukocytes, malignant neoplasm, cancer of breast, medicine, Medicine, Malignancies, Tissue Donor, Medicines, house mouse, mammary cancer, HER2 protein, Breast Malignant Neoplasms, plasma, Tumors, Cellular Immunity, h-BCS, Carcinoma, immunoglobulin secretion, Specialties, Variolations, Transplant Donor, Frozen Plasma, \"breast neoplasm\" EXACT [MTH:120], Process, T-cell surface antigen T4|Leu-3, B Lymphocytes, Immunity, Modern, Transplant, DS, CASP14, mouse, Semen Donors, MLN19, PBMC Peripheral Blood Mononuclear Cells, PBL, Pbl, Plasmas, Medical Specialties., results, Variolation, W91638, Medical Speciality, Benign, NOG, IgG3, IgG4, IgG1, humanised mouse, IgG2, immunoglobulin biosynthetic process, Mini-ICE, Responses, CIT, Specialty, Cellular, Cell-Mediated, NOS, Donors, nog-A, Malignant Neoplasm of Breast, Cell-Mediated Immunities, c-neu, Carcinomas, PTD, Mus musculus, IgG, Caspase-14 subunit p10, Stimulation, mice, p32, T-cell surface glycoprotein CD4, Swiss Mouse, Benign Neoplasms, CD4mut, Caspase-14 subunit p19, HDS, Immunologic, \"mammary neoplasm\" RELATED [], MICE, EGFR2 protein, human, Semen Donor, Malignant Neoplasms, domesticus, 0542/03, MLN 19, Phenotypes, Insurance Medicines, Semen, Cell-Mediated Immunity, hyaline, Patient, breast cancer, Immunities, B-Lymphocyte, human epidermal growth factor receptor 2, IgG2A, Breast Tumors, IgG2B, GT, Breast Malignant Tumors, TKR1, Mouse, Globulins, Immunological Stimulations, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 protein, Polypeptide, cancer, Ovum, Ovum Donors, Organ Donors, 7S, accessory, Fresh, 3.4.22.-, primary breast cancer, CG10439, human being, Cellular Immunities, Fresh Frozen Plasma, BJS, Immunoglobulin, Neoplasms, Benign Neoplasm, number, Mammary Cancers, mini-ICE, CG8114, Immunizations, Malignant, supernumerary, Human, Breast Malignant Tumor, Antibody, Medical Specialities, Antibody Responses, Homo sapiens, GEF, RRG6, House, Breast Tumor, humanized hCYP1A1_1A2_Cyp1a1/1a2(-/-)_Ahr mutant mice, Mus musculus domesticus, \"mammary tumor\" EXACT [CSP2005:2016-0671], breast tumor, HER-2/neu, MAL, Mice, Medical, Man, study, RP59, Blood Plasma, Human Mammary, B-cell, MC3DN1, Neu protein, Malignancy, anon-WO0172774.22, Immunoglobulin GT, IgG(T), Swiss, Mammary Neoplasm, HER-2, Mammary Carcinoma, Antibody Response, D-hil, anon-WO0172774.25, ECT2, CG30147, noggin-1, Cancer of the Breast, Transplant Donors, Neoplasias, GEP6, drugs, Immune Response, Oxaloglycine, Mononuclear Leukocyte, Malignant Tumor of Breast, Clients, Mammary, Mammary Cancer, Blood Plasmas, GRACILE, peptidos, Ovum Donor, Insurance, Breast, Immune Process, mammary tumor, Stimulations, Cancer, Neu, NEU, Organ, Immunologic Sensitization, Breast Carcinoma, Human Mammary Neoplasm, Leukocyte, CPT, Breast Carcinomas, Malignant Neoplasm, Medical Specialty, HER-2|neu, mKIAA3023, 0083/20, Leu2, Production, \"breast tumor\" EXACT [NCI2004_11_17:C2910], Client, Immune Globulins, l(3)09645, Peptide, Cell, Cellular Immune, Impact, Breast Malignant Neoplasm, polypeptide, Environmental, Frozen Plasmas, Immunostimulation, DmelCG8114, MT, Antibody Production, Mus, Mammary Carcinomas, T-cell differentiation antigen L3T4, Neoplasm, Human Mammary Carcinoma, NGL, Donor, Plasma, Peripheral Blood Human Mononuclear Cells, primary cancer, Breast Neoplasm, Immunological Sensitizations, Organ Donor, c-erbB2, increased number, Polyglobin, portion of plasma, House Mice, Bursa-Equivalent Lymphocyte, Cancers, malignant tumor, Immunological, oxalylglycine, Laboratory Mice, present in greater numbers in organism, 0293/09, BPs, 3222401G21Rik, clear, Cellular Immune Response, Modern Man, Breast Cancer, cardinality, 7S Gamma Globulin, Response, Environments, Cellular Immune Responses, CD4, Allerglobuline, Cell Mediated Immunity, Cancer of Breast, Peptid, CD8, Immunologic Stimulation, B-lymphocyte, Immunological Stimulation, Laboratory Mouse, Neoplasia, HER2, Formation, breast"],"pubmed_title_synonyms":["Erbb-2, 3.4.22.-, primary breast cancer, malignant tumor of the breast, CG10439, Laboratory, Neoplasms, Mus domesticus, mammary neoplasm, Mammary Cancers, Human Mammary Neoplasms, mini-ICE, CASP-14, CG8114, nog1, Tumor, 2-oxo-3-azaglutaric acid, House Mouse, Human, CD340, Breast Malignant Tumor, Kiaa3023, RRG6, GEF, \"neoplasm of breast (disorder)\" EXACT [SNOMEDCT_2005_07_31:126926005], House, Breast Tumor, Antigen, Mus musculus domesticus, \"mammary tumor\" EXACT [CSP2005:2016-0671], breast tumor, cancer of the breast, HER-2/neu, Mice, BC, Mammary Neoplasms, malignant neoplasm of breast, Immune Responses, Human Mammary, anon-WO0172774.22, Mammary Neoplasm, Swiss, HER-2, SYM1, Mammary Carcinoma, DmelCG30147, Human Mammary Carcinomas, Swiss Mice, D-hil, anon-WO0172774.25, ECT2, CG30147, SYNS1, Cancer of the Breast, noggin-1, CG13435, GEP6, Immune Response, Oxaloglycine, cancer of breast, Malignant Tumor of Breast, Mammary, Mammary Cancer, Lymphoid, Breast, mammary cancer, mammary tumor, Breast Malignant Neoplasms, Lymphocyte, Neu, NEU, Tumors, Cancer, Breast Carcinoma, Human Mammary Neoplasm, Carcinoma, Breast Carcinomas, \"breast neoplasm\" EXACT [MTH:120], Humoral Immune Response, HER-2|neu, mKIAA3023, CASP14, mouse, 0083/20, MLN19, \"breast tumor\" EXACT [NCI2004_11_17:C2910], PBL, Pbl, Cell, l(3)09645, Breast Malignant Neoplasm, DmelCG8114, Humoral Immunity, NOG, Mammary Carcinomas, Mus, Mini-ICE, Neoplasm, NGL, Human Mammary Carcinoma, NOS, Lymphoid Cell, Humoral, nog-A, Malignant Neoplasm of Breast, Mus musculus., c-neu, Lymphoid Cells, Carcinomas, Mus musculus, Breast Neoplasm, Caspase-14 subunit p10, c-erbB2, mice, Humoral Immune Responses, Swiss Mouse, House Mice, Caspase-14 subunit p19, Cancers, \"mammary neoplasm\" RELATED [], MICE, 0542/03, oxalylglycine, Laboratory Mice, domesticus, MLN 19, 0293/09, Humoral Immune, breast cancer, Breast Tumors, Breast Cancer, Cells, Response, Breast Malignant Tumors, TKR1, Cancer of Breast, Mouse, cancer, Laboratory Mouse, HER2, breast"],"description_synonyms":["liquid chromatography tandem mass spectroscopy, LC-MS2, 3.4.22.-, human being, Peptidomics, Laboratory, protein complex, Blood, Modern, Mus domesticus, Proteins, mouse, LC-MS/MS, CASP14, Gene, mini-ICE, CASP-14, protein, Blood Serum, Serum, protein-containing complex, House Mouse, LC-MS-MS, Human, LC/MS/MS, native protein, Homo sapiens, House, Mus, Protein, LC-MSMS, Mini-ICE, Gene Products, Mus musculus domesticus, protein aggregate, Mice, Man, Mus musculus, study, 10^[-9], LCMSMS, Man (Taxonomy), Caspase-14 subunit p10, Swiss, mice, Swiss Mouse, Blood., House Mice, Swiss Mice, Caspase-14 subunit p19, MICE, human, domesticus, Laboratory Mice, Protein Gene Products, Gene Proteins, liquid chromatography-tandem mass spectroscopy, Modern Man, n, liquid chromatography tandem mass spectrometry, Mouse, house mouse, Laboratory Mouse, Serums"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics of Human and Mouse serum protein","description":"Proteomics of human and mouse serum protein using nano-LC-MS/MS.  In this study, humanized mice serum were included.","dates":{"publication":"Tue Sep 15 00:00:00 BST 2020"},"accession":"PXD021477","cross_references":{"TAXONOMY":["10090","9606"],"pubmed":["34140620"]}}