JPOST 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MingliangHomo Sapiens (human)https://repository.jpostdb.org/entry/JPST000965nanyang technological universityjPOSTsodium salt, 2-ethylhexyl hydrogen phthalate, 2-Ethylhexyl phthalate, 2-ethylhexyl ester, mono(2-ethylhexyl)phthalate, 2-([(2-ethylhexyl)oxy]carbonyl)benzoic acid, MEHP, results., mono-(2-ethylhexyl)phthalate, monoethylhexyl phthalate, MEHP cpd, 1, (2-ethylhexyl) hydrogen phthalate, mono(2-ethylhexyl) ester, mono-2-ethylhexyl phthalate, 2-benzenedicarboxylic acid, mono(ethylhexyl) phthalate, phthalic acidsodium salt, biochemical pathways, single-organism catabolic process, multicellular organismal catabolic process, acido graso, 2-Ethylhexyl phthalate, SDR5C1, xspr, acidos grasos, determination, MEHP cpd, Lipidomic, MRPP2, Blood, Hadhsc, Di 2 Ethylhexylphthalate, 17bHSD10, neutral molecular compounds, ABAD, potassium hydrogen phthalate, dmTAF[[II]]230, Techniques, Roles, Method, 1, Dioctyl, Concepts, cellular degradation, Saturated Fatty Acids, multicellular organism lipid catabolic process, Esterified Fatty Acid, ECHA, molecule, integumentum commune, Esterified, molecula, imprinted and ancient gene protein, treatment, Fresh Frozen Plasmas, Fresh Frozen, molecules, TFIID TAF250, enzymes, pre-mortem, cel, catabolism, Esterified Fatty, Di(2-ethylhexyl)phthalate, Aliphatic, Hadh2, Phthalic acid bis(2-ethylhexyl) ester, DUPXp11.22, proteins, Molekuel, SDR38C1, AW742602, Di(2-ethylhexyl)orthophthalate, 2-([(2-ethylhexyl)oxy]carbonyl)benzoic acid, Aliphatic Acids, di-iso-Octyl phthalate, Methodological Studies, HAD, scientific observation, disease management, Therapies, biotransformation, mono(2-ethylhexyl) ester, Diethylhexyl phthalate, LCEH, plasma, monoiron (2+) salt, 1.1.1.153, HADH2, Therapy, HADH1, MEHP, dTAF[[II]]230, Frozen Plasma, HCD2, external covering of organism, TAC1R, TAF200, MRX31, organism surface, Bis(ethylhexyl) phthalate, Fettsaeuren, Procedure, TAFII-250, TAF250/230, NK1R, Tac1r, Plasmas, CAMR, results, HCDH, monolead (2+) salt, AU019341, multicellular organismal lipid catabolic process, TAFII250, Role Concept, SCHAD, Lipid, Role, secretion, Acid, portion of blood plasma, Fatty Acid, breakdown, Saturated Fatty Acid, disodium salt, Aliphatic Acid, GBP, Methodological, lipid biosynthesis, CG17603, MRX17, Methodological Study, TAF[[II]], Schad, Nk1r, Treatments, monosodium salt, HADHSC, living, Lipidome, fatty acids, blood plasm, Taf250, 2-(2-ethylhexyloxycarbonyl)benzoic acid, integumentary system, SR3-5, copper salt, Biocatalyst, Di-2-Ethylhexylphthalate, (2-ethylhexyl) hydrogen phthalate, ENSMUSG00000071345, monobarium salt, E430016J11Rik, TP-ALPHA, dermal system, TAF230, Saturated Fatty, Fresh, lipids, Esterified Fatty Acids, d230, Procedures, Fresh Frozen Plasma, Biocatalysts, Gene, dTAFII250, mono-2-ethylhexyl phthalate, Ximpact, EfW1, HADH, cellular catabolism, Lipolyses, mono(2-ethylhexyl)phthalate, phthalate, method, surface, potassium salt, mono-(2-ethylhexyl)phthalate, acides gras, Bis(2-ethylhexyl) o-phthalate, dmTAF1, 2-Benzenedicarboxylic acid, Taf230, method used in an experiment, Dioctyl phthalate, Gene Products, Studies, lipid anabolism, Role Concepts., 2-benzenedicarboxylic acid, MHBD, Technique, Di(ethylhexyl) phthalate, TAF250, Fatty Acids, Taf200, Blood Plasma, dTAF[[II]]250, monoruthenium salt, Phthalate, breakdown of chemical, cell, ligand, Taf1p, DEHP, Gm10328, mono(ethylhexyl) phthalate, Study, dTAF250, AA409688, Enzyme, monoethylhexyl phthalate, dipotassium salt, Blood Plasmas, MRXS10, bis(2-ethylhexyl)phthalate, TAF, Bis(2-ethylhexyl)phthalate, Di-sec-octyl phthalate, Dioctyl Phthalate, cellular breakdown, measuring, NKIR, Saturated, TAF[[II]]250, lipogenesis, degradation, HHF4, Proteins, Lipidomes, l(3)84Ab, SPR, Spr, enzyme activity, BG:DS00004.13, Diethylhexyl, impact-a, Cell, dTAF230, Concept, Frozen Plasmas, Fettsaeure, Di(2-ethylhexyl) o-phthalate, p230, Protein, chemical analysis, ERAB, TAF[[II]]250/230, IMPACT, imprinted and ancient gene protein homolog, TFIID, Plasticizer, Ads9, monocalcium salt, phthalic acid, breakdown of molecule, Taf[[II]]250, Plasma, potassium, bis(2-ethylhexyl) ester, TAF[[II]]230, disodium phthalate, lipid formation, biodegradation, body surface, lipid synthesis, portion of plasma, TAF[II]250, acide gras, monopotassium salt, LCHAD, Protein Gene Products, plan specification, 2-ethylhexyl hydrogen phthalate, 2-ethylhexyl ester, Gene Proteins, 17b-HSD10, breakdown of substance, DmelCG17603, AA409008, Therapeutic, Phthalic acid di(2-ethylhexyl) ester, Treatment, assay, Octyl phthalate, MSCHAD, 2-Benzenedicarboxylic acid bis(2-ethylhexyl) ester, RWDD5, MTPA, cellular lipid metabolism, TAF1falseMEHP CETSA results for targets screeningMonoethylhexyl phthalate (MEHP) is one of the main active metabolites of the plasticizer di(2-ethylhexyl) phthalate (DEHP). It has been known that MEHP has impact on lipolysis; however, its mechanism on the cellular lipid metabolism remains largely unclear. Here, we first utilized the global lipid profiling to fully characterize the lipid synthesis and degradation pathways upon MEHP treatment. Meanwhile, we further identified the possible MEHP targeted proteins in the living cells using the cellular thermal shift assay (CETSA) method. The lipidomics result showed that there was a significant accumulation of fatty acids and other lipids in cell. The CETSA identified 138 proteins and fatty acid β-oxidation inhibition pathways that were significantly perturbed. MEHP’s binding with selected proteins HADH and HSD17B10 was further evaluated using molecule docking and results showed that MEHP has higher affinities as compared to the endogenous substrates, which was further experimentally confirmed in the surface plasma resonance (SPR) interaction assay. In summary, we found a novel mechanism for MEHP-induced lipid accumulation, which was probably due to its inhibitive effects on the enzymes in fatty acid β-oxidation. This mechanism substantiates the public concerns on the high exposure level to plasticizers and its possible role as an obesogen.Sat Oct 16 00:00:00 BST 2021PXD0220349606